Publication
Survival of clinical and environmental carbapenem-resistant Klebsiella pneumoniae ST147 in surface water
| dc.contributor.author | Ferreira, Catarina | |
| dc.contributor.author | Luzietti, Lara | |
| dc.contributor.author | Ribeirinho-Soares, Sara | |
| dc.contributor.author | Nunes, Olga C. | |
| dc.contributor.author | Vaz-Moreira, Ivone | |
| dc.contributor.author | Manaia, Célia M. | |
| dc.date.accessioned | 2023-09-11T18:25:06Z | |
| dc.date.available | 2023-09-11T18:25:06Z | |
| dc.date.issued | 2023-11-15 | |
| dc.description.abstract | Carbapenem-resistant Klebsiella pneumoniae represents a healthcare threat, already disseminated in the environment. This study aimed to compare the behaviour of a clinical and an environmental K. pneumoniae strain (multilocus sequence type ST147) harbouring the gene blaKPC-3 in water. The abundance of the genes phoE (specific for K. pneumoniae) and blaKPC-3 was monitored by quantitative PCR in urban runoff water and sterile ultra-pure water microcosms, aiming to assess survival, blaKPC-3 persistence, and the effect of the native water microbiota. In sterile ultra-pure water, the abundance of cultivable K. pneumoniae and blaKPC-3 gene did not change over the incubation period (8 days). In contrast, in urban runoff, the K. pneumoniae and the genes phoE and blaKPC genes decreased by up to 3 log-units. These results suggest that K. pneumoniae were outcompeted by the native microbiota of the urban runoff water and that the decay of blaKPC-3 gene was due to host death, rather than to gene loss. The study highlights that although native microbiota is essential to hamper the persistence of non-native bacteria, carbapenemase producing K. pneumoniae can survive in urban runoff water for at least one week. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1016/j.envres.2023.116928 | pt_PT |
| dc.identifier.eid | 85168809588 | |
| dc.identifier.issn | 0013-9351 | |
| dc.identifier.pmid | 37607624 | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/42257 | |
| dc.identifier.wos | 001065180700001 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Bacteria survival | pt_PT |
| dc.subject | Microcosms | pt_PT |
| dc.subject | Competition | pt_PT |
| dc.subject | Native microbiota | pt_PT |
| dc.title | Survival of clinical and environmental carbapenem-resistant Klebsiella pneumoniae ST147 in surface water | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.title | Environmental Research | pt_PT |
| oaire.citation.volume | 237 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |