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Chitosan increases Pinus pinaster tolerance to the pinewood nematode (Bursaphelenchus xylophilus) by promoting plant antioxidative metabolism

2021-12Journal article Open access
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dc.contributor.authorSilva, Marta Nunes da
dc.contributor.authorSantos, Carla S.
dc.contributor.authorCruz, Ana
dc.contributor.authorLópez-Villamor, Adrián
dc.contributor.authorVasconcelos, Marta W.
dc.date.accessioned2021-03-26T15:36:57Z
dc.date.available2021-03-26T15:36:57Z
dc.date.issued2021-12
dc.description.abstractThe pine wilt disease (PWD), for which no effective treatment is available at the moment, is a constant threat to Pinus spp. plantations worldwide, being responsible for significant economic and environmental losses every year. It has been demonstrated that elicitation with chitosan increases plant tolerance to the pinewood nematode (PWN) Bursaphelenchus xylophilus, the causal agent of the PWD, but the biochemical and genetic aspects underlying this response have not been explored. To understand the influence of chitosan in Pinus pinaster tolerance against PWN, a low-molecular-weight (327 kDa) chitosan was applied to mock- and PWN-inoculated plants. Nematode population, malondialdehyde (MDA), catalase, carotenoids, anthocyanins, phenolic compounds, lignin and gene expression related to oxidative stress (thioredoxin 1, TRX) and plant defence (defensin, DEF, and a-farnesene synthase, AFS), were analysed at 1, 7, 14, 21 and 28 days post-inoculation (dpi). At 28 dpi, PWN-infected plants elicited with chitosan showed a sixfold lower nematode population when compared to non-elicited plants. Higher levels of MDA, catalase, carotenoids, anthocyanins, phenolic compounds, and lignin were detected in chitosan-elicited plants following infection. The expression levels of DEF gene were higher in elicited plants, while TRX and AFS expression was lower, possibly due to the disease containment-effect of chitosan. Combined, we conclude that chitosan induces pine defences against PWD via modulation of metabolic and transcriptomic mechanisms related with plant antioxidant system.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-021-83445-0pt_PT
dc.identifier.eid85100869239
dc.identifier.issn2045-2322
dc.identifier.pmcPMC7881030
dc.identifier.pmid33580134
dc.identifier.urihttp://hdl.handle.net/10400.14/32338
dc.identifier.wos000620252500023
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleChitosan increases Pinus pinaster tolerance to the pinewood nematode (Bursaphelenchus xylophilus) by promoting plant antioxidative metabolismpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume11pt_PT
person.familyNameNunes da Silva
person.familyNameSancho dos Santos
person.familyNameLopez Villamor
person.familyNameVasconcelos
person.givenNameMarta
person.givenNameCarla
person.givenNameAdrian
person.givenNameMarta
person.identifier211408
person.identifier74696
person.identifier.ciencia-id3C1B-7E7B-F695
person.identifier.ciencia-idF715-AACD-9820
person.identifier.ciencia-id0216-882C-3070
person.identifier.orcid0000-0001-8228-0576
person.identifier.orcid0000-0002-6708-5550
person.identifier.orcid0000-0001-7822-0538
person.identifier.orcid0000-0002-5110-7006
person.identifier.ridI-8166-2013
person.identifier.scopus-author-id55576995600
person.identifier.scopus-author-id14627797100
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb7e51c98-600e-42ac-9dd6-0d70e8fc5f19
relation.isAuthorOfPublication00a4c1fc-814e-400c-8bfd-4680cff6deb1
relation.isAuthorOfPublication08a21780-c424-4c27-84b1-13a0acaa12f2
relation.isAuthorOfPublicationdf60ebb4-3b71-4733-a9e3-bfa4687f5576
relation.isAuthorOfPublication.latestForDiscovery00a4c1fc-814e-400c-8bfd-4680cff6deb1

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