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Development and Optimization of an Online SPE-HPLC-FD Method for Quantification of Fluoroquinolones in Wastewater Effluents

dc.contributor.authorMeira, Bernardo
dc.contributor.authorMaia, Alexandra S.
dc.contributor.authorGonçalves, Virgínia M. F.
dc.contributor.authorRibeiro, Ana R.
dc.contributor.authorTiritan, Maria E.
dc.date.accessioned2013-11-27T18:19:47Z
dc.date.available2013-11-27T18:19:47Z
dc.date.issued2013
dc.description.abstractFluoroquinolones are antimicrobial agents widely found in environmental matrices and extensively studied due to their persistence and implications for multiresistant bacteria. The presence of fluoroquinolones in the environment is mainly due to the incapability of wastewater treatment plants (WWTPs) to completely remove those compounds. The amount of fluoroquinolones released through effluents depends on the type of treatment used by the WWTPs. So, accurate analytical methods to quantify those compounds on WWTPs process and in effluents are crucial. Solid phase extraction (SPE) coupled to liquid chromatography is a straightforward technique that provides analyte extraction, cleanup, separation and detection while providing a good reproducibility and efficiency. The purpose of this work was the establishment of a novel method for quantification of Ofloxacin, Norfloxacin, Ciprofloxacin and Moxifloxacin on WWTPs effluents using on-line SPE. Samples were injected directly on a restricted access material column LichroCart 25-4 Lichrospher® RP-18 ADS (25 μm) and then transferred to an analytical column Luna PFP (2) (150 x 4.6 mm ID, 100 Å, 3 μm) for separation in isocratic mode with a mixture of 0.1% triethylamine in water (acidified to pH = 2.2 with trifluoroacetic acid) and ethanol as mobile phase; column oven was set at 45ºC. The detection was performed by fluorescence with an excitation wavelength of 290 nm and an emission wavelength of 460 nm. The injection volume of 100 μL of previous preconcentrated sample was compared with larger volume injection of only filtered effluent samples. The study was conducted with effluent samples collected from a municipal WWTP in the north of Portugal.por
dc.identifier.citationMEIRA, Bernardo [et al.] - Development and Optimization of an Online SPE-HPLC-FD Method for Quantification of Fluoroquinolones in Wastewater Effluents. In 39th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2013), Amsterdam, Netherlands, 16-20 June 2013 – In HPLC 2013: Book of Abstracts. 2013. p.390por
dc.identifier.urihttp://hdl.handle.net/10400.14/13279
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectFluoroquinolonespor
dc.subjectOnline SPE-HPLCpor
dc.titleDevelopment and Optimization of an Online SPE-HPLC-FD Method for Quantification of Fluoroquinolones in Wastewater Effluentspor
dc.typeconference object
dspace.entity.typePublication
person.familyNameMaia
person.familyNameTiritan
person.givenNameAlexandra S.
person.givenNameMaria Elizabeth
person.identifier1310409
person.identifier.ciencia-id6A15-F0A3-AEF2
person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.orcid0000-0002-4167-383X
person.identifier.orcid0000-0003-3320-730X
person.identifier.ridK-9492-2014
person.identifier.scopus-author-id55332349300
person.identifier.scopus-author-id6602890981
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublication56eedbbf-8105-4d55-bf1f-e38c62ed4da5
relation.isAuthorOfPublication1ab8e8f5-cf82-4edd-89d8-46e5cca13e19
relation.isAuthorOfPublication.latestForDiscovery1ab8e8f5-cf82-4edd-89d8-46e5cca13e19

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