Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages

Amorim, Lara R. P.; Silva, Joana G. L.; Gibbs, Paul A.; Teixeira, Paula C.

Publication

Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages

2009Journal article

dc.contributor.author	Amorim, Lara R. P.
dc.contributor.author	Silva, Joana G. L.
dc.contributor.author	Gibbs, Paul A.
dc.contributor.author	Teixeira, Paula C.
dc.date.accessioned	2010-11-02T14:27:30Z
dc.date.available	2010-11-02T14:27:30Z
dc.date.issued	2009
dc.description.abstract	This study was performed to evaluate the adaption of the impedimetric method to detect the lytic infection by Salmonella-specific bacteriophages and to provide a higher selectivity to this rapid method in detecting Salmonella spp. by using specific agents. Three bacteriophages and twelve strains of Salmonella spp. were tested. Each of the twelve strains was used separately to inoculate TSB together with each one of the phages. The inoculumconcentration was between 106 and 107 cfu/mL, at a cell: phage ratio of 1 : 100. From the sample analysis, based on conductance (G)measurements (37◦C), the infection could be detected, by observation of both detection-time delay and distinct curve trends. The main conclusions were that kinetic detection by impedance microbiology with phage typing constitutes a method of determining whether a test microorganism is sensitive to the bacteriophage and a method to evaluate whether a lytic bacteriophage is present in a sample, by affecting bacterial growth rate/metabolic change	por
dc.description.version	info:eu-repo/semantics/publishedVersion
dc.identifier.citation	AMORIM, Lara R. P ...[et al.] - Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages. International Journal of Microbiology. ISSN 1687-918X. (2009), p. 1-6	por
dc.identifier.doi	10.1155/2009/259456
dc.identifier.uri	http://hdl.handle.net/10400.14/3258
dc.language.iso	eng	por
dc.publisher	Hindawi Publishing Corporation	por
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.title	Application of an impedimetric technique for the detection of lytic infection of salmonella spp. by specific phages	por
dc.type	journal article
dspace.entity.type	Publication
person.familyName	Gibbs
person.familyName	Teixeira
person.givenName	Paul
person.givenName	Paula
person.identifier.orcid	0000-0002-6580-4363
person.identifier.orcid	0000-0002-6296-5137
person.identifier.rid	J-8678-2014
person.identifier.scopus-author-id	7005895206
rcaap.rights	openAccess	por
rcaap.type	article	por
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