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Population wide testing pooling strategy for SARS-CoV-2 detection using saliva

2022-01-28Journal article Open access
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dc.contributor.authorEsteves, Eduardo
dc.contributor.authorMendes, Ana Karina
dc.contributor.authorBarros, Marlene
dc.contributor.authorFigueiredo, Cátia
dc.contributor.authorAndrade, Joana
dc.contributor.authorCapelo, Joana
dc.contributor.authorNovais, António
dc.contributor.authorRebelo, Carla
dc.contributor.authorSoares, Rita
dc.contributor.authorNunes, Ana
dc.contributor.authorFerreira, André
dc.contributor.authorLemos, Joana
dc.contributor.authorDuarte, Ana Sofia
dc.contributor.authorSilva, Raquel M.
dc.contributor.authorBernardino, Liliana Inácio
dc.contributor.authorCorreia, Maria José
dc.contributor.authorEsteves, Ana Cristina
dc.contributor.authorRosa, Nuno
dc.date.accessioned2022-02-03T15:17:38Z
dc.date.available2022-02-03T15:17:38Z
dc.date.issued2022-01-28
dc.description.abstractSARS-CoV-2 pandemic has forced frequent testing of populations. It is necessary to identify the most cost-effective strategies for the detection of COVID-19 outbreaks. Nasopharyngeal samples have been used for SARS-CoV-2 detection but require a healthcare professional to collect the sample and cause discomfort and pain to the individual. Saliva has been suggested as an appropriate fluid for the diagnosis of COVID-19. We have investigated the possibility of using pools of saliva samples to detect SARS-CoV-2 in symptomatic and asymptomatic patients. Two hundred and seventy-nine saliva samples were analyzed through RT-PCR of Envelope, Nucleocapsid and Open Reading Frame 1ab genes. Reproducibility assays showed an almost perfect agreement as well as high sensitivity (96.6%), specificity (96.8%), positive predicted value (96.6%), and negative predicted value (96.8%). The average Cycle Threshold of the genes detected was 29.7. No significant differences (p > 0.05) were detected when comparing the cycle threshold average of two consecutive reactions on the same positive saliva samples. Saliva samples have a higher median viral load (32.6) than in nasopharyngeal samples (28.9), although no significant differences were detected (p > 0.05). Saliva-pool samples allowed effective SARS-CoV-2 screening, with a higher sensibility (96.9%) on 10-sample pools than in 20-sample pools (87.5%). Regardless of pools size specificity was high (99.9%) and an almost perfect agreement was observed. Our strategy was successfully applied in population wide testing of more than 2000 individuals, showing that it is possible to use pooled saliva as diagnostic fluid for SARS-CoV-2 infection.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1371/journal.pone.0263033pt_PT
dc.identifier.eid85123695256
dc.identifier.issn1932-6203
dc.identifier.pmcPMC8797214
dc.identifier.pmid35089942
dc.identifier.urihttp://hdl.handle.net/10400.14/36588
dc.identifier.wos000769158400035
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titlePopulation wide testing pooling strategy for SARS-CoV-2 detection using salivapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume17pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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