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Micro-PAD card for measuring total ammonia nitrogen in saliva

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dc.contributor.authorThepchuay, Yanisa
dc.contributor.authorMesquita, Raquel
dc.contributor.authorNacapricha, Duangjai
dc.contributor.authorRangel, António O. S. S.
dc.date.accessioned2020-04-27T14:41:25Z
dc.date.available2020-04-27T14:41:25Z
dc.date.issued2020
dc.description.abstractThis work presents a portable microfluidic paper-based analytical device (micro-PAD) card for the quantification of total ammonia nitrogen in human saliva. The amount of total ammonia nitrogen in saliva can be an indicator of the status of the oral microbiome with potential correlation to kidney health problems. The developed micro-PAD card comprises twenty units consisting of three stacked layers of circular discs: the sample layer, paper discs impregnated with sodium hydroxide solution, the PTFE membrane layer, and the detection layer, paper discs impregnated with bromothymol blue. The twenty units were aligned on transparent laminating pouches laminated to form the micro-PAD card (7.5 cm × 10.5 cm). Saliva samples can be directly dispensed onto the micro-PAD card and the detection was achieved by the BTB indicator color change, from yellow to blue, after conversion of ammonium into ammonia and diffusion of the ammonia gas through a hydrophobic layer. The determination of total ammonia nitrogen in saliva using the developed micro-PAD card intended to be very simple method and operated without the need of laboratory equipment. A quantification limit of 11.3 NH4+mg L-1 and linear application range from up to 150 NH4+mg L-1 were obtained making it suitable for the expected concentrations of total ammonia nitrogen in human saliva. It was successfully applied to saliva samples and its validation obtained by comparison against a potentiometric method. The card is stable for at least 1 month making it ideal as a portable device for point-of-care diagnosis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationThepchuay, Y., Mesquita, R. B., Nacapricha, D., Rangel, A. O. (2020). Micro-PAD card for measuring total ammonia nitrogen in saliva. Analytical and Bioanalytical Chemistrypt_PT
dc.identifier.doi10.1007/s00216-020-02577-wpt_PT
dc.identifier.eid85083859497
dc.identifier.eissn1618-2650
dc.identifier.issn1618-2642
dc.identifier.pmid32303795
dc.identifier.urihttp://hdl.handle.net/10400.14/30417
dc.identifier.wos000530622800013
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationCentre for Biotechnology and Fine Chemistry
dc.subjectTotal ammonia nitrogenpt_PT
dc.subjectBromothymol bluept_PT
dc.subjectMicrofluidic paper-based analytical devicept_PT
dc.subjectSalivapt_PT
dc.subjectMicro-PADpt_PT
dc.titleMicro-PAD card for measuring total ammonia nitrogen in salivapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2019/PT
oaire.citation.titleAnalytical and Bioanalytical Chemistrypt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMesquita
person.familyNameSantos Silva Rangel
person.givenNameRaquel
person.givenNameAntónio Osmaro
person.identifierJ-4630-2013
person.identifier.ciencia-idEB1D-DEE3-0B60
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.orcid0000-0001-7745-1860
person.identifier.orcid0000-0002-6486-8947
person.identifier.ridJ-4630-2013
person.identifier.scopus-author-id7005556626
person.identifier.scopus-author-id7004827387
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf89887f1-4d1b-438b-8f03-27d9bda1de7b
relation.isAuthorOfPublication467be022-fd9c-4549-aa48-48ebcc1098eb
relation.isAuthorOfPublication.latestForDiscovery467be022-fd9c-4549-aa48-48ebcc1098eb
relation.isProjectOfPublication1f76a7db-2520-482f-b56b-7e01ea36dd1f
relation.isProjectOfPublication.latestForDiscovery1f76a7db-2520-482f-b56b-7e01ea36dd1f

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