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Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters

2001Journal article Open access
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dc.contributor.authorRamalho, Rita
dc.contributor.authorCunha, Joaquim
dc.contributor.authorTeixeira, Paula
dc.contributor.authorGibbs, Paul A.
dc.date.accessioned2011-10-22T16:25:24Z
dc.date.available2011-10-22T16:25:24Z
dc.date.issued2001
dc.description.abstractAt this time the European Union regulations require that the heterotrophic plate counts (HPC) of mineral waters be assessed at two recovery temperatures: 22°C for 72 h and 37°C for 24 h. This procedure is time consuming and expensive. Development of new rapid methods for microbiological assessment of the microbial flora in the bottled water is an industry-driven need. The objectives of this work were to develop a method for the HPC that utilises only one recovery temperature and one incubation period and evaluate the use of, the LIVE/DEAD® BacLight™ Bacterial Viability Kit, 5-cyano-2,3-ditotyl tetrazolium chloride (CTC) and impedance methods to enumerate viable bacteria in bottled mineral water. Results showed that incubation at 30°C could be used instead of incubation at 22°C and 37°C. Good correlation exists between counts at 30°C and counts at 22°C (r>0.90) and all the pathogens important in mineral water analyses grow similarly at 30°C and 37°C during 24 h. It was demonstrated that impedance methods might be useful to the mineral water industry as a rapid indicator of microbiological quality of the water. Results obtained with BacLight and CTC were similar to those obtained with plate counts.por
dc.identifier.citationRAMALHO, Rita ...[et al.] - Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters. Journal of Microbiological Methods. ISSN 0167-7012. Vol. 44, n.º2 (2001), p. 97–103por
dc.identifier.urihttp://hdl.handle.net/10400.14/6841
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversiondoi:10.1016/S0167-7012(00)00237-2por
dc.subjectBacLightpor
dc.subjectCTCpor
dc.subjectHeterotrophic plate countspor
dc.subjectImpedance methodspor
dc.subjectMineral waterpor
dc.titleImproved methods for the enumeration of heterotrophic bacteria in bottled mineral waterspor
dc.typejournal article
dspace.entity.typePublication
person.familyNameTeixeira
person.familyNameGibbs
person.givenNamePaula
person.givenNamePaul
person.identifier.orcid0000-0002-6296-5137
person.identifier.orcid0000-0002-6580-4363
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id7005895206
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isAuthorOfPublication218d85c8-40f4-42c0-9a47-4e8ad9f72a96
relation.isAuthorOfPublication.latestForDiscovery218d85c8-40f4-42c0-9a47-4e8ad9f72a96

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