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Optimization of enzyme-assisted extraction of bioactive compounds from sea buckthorn (Hippophae rhamnoides L.) leaves: evaluation of mixed-culture fermentation

2023-09-29Journal article Open access
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dc.contributor.authorPuzeryte, Viktorija
dc.contributor.authorMartusevice, Paulina
dc.contributor.authorSousa, Sérgio
dc.contributor.authorBalciunaitiene, Aiste
dc.contributor.authorViskelis, Jonas
dc.contributor.authorGomes, Ana Maria
dc.contributor.authorViskelis, Pranas
dc.contributor.authorCesoniene, Laima
dc.contributor.authorUrbonaviciene, Dalia
dc.date.accessioned2023-10-12T16:34:35Z
dc.date.available2023-10-12T16:34:35Z
dc.date.issued2023-09-29
dc.description.abstractHippophae rhamnoides L. leaves possess a remarkable amount of polyphenols that could serve as a natural remedy in various applications. In comparison, numerous techniques, such as conventional and high-pressure techniques, are available for extracting the bioactive fractions from sea buckthorn leaves (SBL). However, enzyme-assisted extraction (EAE) of SBL has not been comprehensively studied. The aim of this study was to optimize critical EAE parameters of SBL using the cellulolytic enzyme complex, Viscozyme L, to obtain a high-yield extract with a high concentration of bioactive compounds. In order to determine the optimal conditions for EAE, the study employed a central composite design and response surface methodology to analyze the effects of four independent factors (pH, temperature, extraction time, and enzyme concentration) on two different responses. Our findings indicated that under optimal conditions (3:15 h extraction, temperature 45 °C, pH 4.9, and 1% Viscozyme L v/w of leaves DW), EAE yielded 28.90 g/100 g DW of the water-soluble fraction. Furthermore, the EAE-optimized liquid extract was continuously fermented using an ancient fermentation starter, Tibetan kefir grains, which possess lactic acid bacteria (LAB) and have significant potential for use in biopreservation. Interestingly, the results indicated various potential prebiotic characteristics of LAB. Additionally, alterations in the cell wall morphology of the SBL residue after EAE were examined using scanning electron microscopy (SEM). This study significantly optimized EAE parameters for sea buckthorn leaves, providing a promising natural source of bioactive compounds for various applications, such as nutraceuticals, functional foods, and high-value products.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/microorganisms11092180pt_PT
dc.identifier.eid85172759334
dc.identifier.issn2076-2607
dc.identifier.pmcPMC10536544
dc.identifier.pmid37764024
dc.identifier.urihttp://hdl.handle.net/10400.14/42849
dc.identifier.wos001076723900001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCentral composite designpt_PT
dc.subjectEnzyme-assisted extractionpt_PT
dc.subjectFermentationpt_PT
dc.subjectHippophae rhamnoidesLpt_PT
dc.subjectOptimizationpt_PT
dc.subjectSea buckthorn leavespt_PT
dc.titleOptimization of enzyme-assisted extraction of bioactive compounds from sea buckthorn (Hippophae rhamnoides L.) leaves: evaluation of mixed-culture fermentationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue9pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume11pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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