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Methacrylated epigallocatechin gallate functionalized dental adhesives: antiproteolytic activity and dentin bonding studies

2025-07-01Artigo de investigação Acesso aberto
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dc.contributor.authorDuque, Cristiane
dc.contributor.authorChrisostomo, Daniela Alvim
dc.contributor.authorScaffa, Polliana Mendes Candia
dc.contributor.authorGouveia, Zach
dc.contributor.authorNascimento, Fabio Dupart
dc.contributor.authorPlotnikov, Sergey V.
dc.contributor.authorPrakki, Anuradha
dc.date.accessioned2025-08-14T07:42:03Z
dc.date.available2025-08-14T07:42:03Z
dc.date.issued2025-07-01
dc.description.abstractObjectives: To assess the antiproteolytic effect of EGCG-methacrylate monomers and its inhibitory effect on gelatinolytic activity in the hybrid layer. Also, to investigate the effect of an adhesive material functionalized with EGCG-methacrylate monomers on immediate and long-term dentin-resin bond strength. Methods: Neat EGCG (E0) was reacted with three different ratios of methacryloyl ester and dissolved in ethyl acetate to obtain EGCG-methacrylates with hydroxyl functionalization at 33 % (M-E33), 67 % (M-E67) and 100 % (M-E100) levels. Resin composite blocks were built on human dentin surfaces using self-etching adhesive containing E0, M-E33, M-E67, and M-E100 at 1 wt%. Demineralized human dentin disks were immersed in deionized water (DW) or lactic acid (LA) and subsequently treated with DW, acetone (as controls), E0, M-E33, M-E67 and M-E100 diluted in acetone. Concentrations of solubilized type I collagen C-terminal (CTX and ICTP) and N-terminal (NTX) telopeptides were determined from 7-day extracts of dentin matrix specimens by ELISA assays. In situ zymography of adhesive-dentin interface slices from restored teeth was performed by confocal microscope after 24 h dentin treatment. Microtensile bond strength (µTBS) and failure pattern were evaluated after 24 h and 6 months. Data were analyzed using two-way ANOVA and Tukey post hoc test (p < 0.05). Results: All experimental groups statistically reduced the release of solubilized telopeptides from dentin samples in DW and LA. E0 and M-E100 incorporated into the adhesive system reduced the gelatinolytic activity within the hybrid layer. The lowest µTBS values for restored teeth were observed for E0 and M-E100 groups, after 24 h and 6 months, respectively. The most prevalent failure observed was classified as type 4, except for M-E100. Significance: EGCG-methacrylate monomers effectively protected collagen from degradation. When incorporated into adhesive systems, EGCG-methacrylates reduced gelatinolytic activity within the hybrid layer, and did not affect immediate and long-term bond strength values of restorations.eng
dc.identifier.citationDuque, C., Chrisostomo, D. A., Scaffa, P. M. C., & Gouveia, Z. et al. (2025). Methacrylated epigallocatechin gallate functionalized dental adhesives: antiproteolytic activity and dentin bonding studies. Dental Materials, 41(7), 892-900. https://doi.org/10.1016/j.dental.2025.05.006
dc.identifier.doi10.1016/j.dental.2025.05.006
dc.identifier.eid105007063167
dc.identifier.issn0109-5641
dc.identifier.other282764be-8b5c-47c8-aa86-f86a65de024d
dc.identifier.pmid40467428
dc.identifier.urihttp://hdl.handle.net/10400.14/54574
dc.identifier.wos001508912600010
dc.language.isoeng
dc.peerreviewedyes
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAdhesives
dc.subjectEGCG
dc.subjectHuman dentin
dc.subjectMetalloproteinases
dc.subjectMethacrylates
dc.titleMethacrylated epigallocatechin gallate functionalized dental adhesives: antiproteolytic activity and dentin bonding studieseng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.endPage900
oaire.citation.issue7
oaire.citation.startPage892
oaire.citation.titleDental Materials
oaire.citation.volume41
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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