Publicação
Evaluation of mini-VIDAS rapid test for detection of Listeria monocytogenes from production lines of fresh to cold-smoked fish
| dc.contributor.author | Vaz-Velho, Manuela | |
| dc.contributor.author | Duarte, Gabriela | |
| dc.contributor.author | Gibbs, Paul | |
| dc.date.accessioned | 2011-10-22T12:19:16Z | |
| dc.date.available | 2011-10-22T12:19:16Z | |
| dc.date.issued | 2000 | |
| dc.description.abstract | This study was conducted to evaluate the efficacy of the mini-VIDAS Listeria monocytogenes (LMO) system (BioMe´rieux Vitek, Inc., Missouri, USA) for detection of L. monocytogenes in environmental and fish samples from three Portuguese cold-smoking plants and from their fresh fish suppliers. Mini-VIDAS-LMO is a fully automated system that uses fluorescent ELFA (Enzyme Linked Fluorescent Assay) technology for detection of Listeria monocytogenes antigens in food. It can be a rapid screening method alternative to time consuming classical isolation and identification. Two hundred and ninety five samples were tested in mini-VIDAS-LMO and in parallel by the ISO 11290-1 traditional protocol. The mini-VIDAS-LMO detected 8 of the 11 confirmed positive samples and presented 11 false positive results. The specificity of the mini-VIDASLMO found in this experiment was 0.96 and the sensitivity 0.73 | por |
| dc.identifier.citation | VAZ-VELHO, Manuela; DUARTE, Gabriela; GIBBS, Paul - of Listeria monocytogenes from production lines of fresh to cold-smoked fish. Journal of Microbiological Methods. ISSN 0167-7012. Vol. 40 (2000), p. 147–151 | por |
| dc.identifier.doi | 10.1016/S0167-7012(00)00118-4 | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/6809 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.subject | Cold-smoked fish | por |
| dc.subject | Listeria monocytogenes | por |
| dc.subject | Rapid immunological-based methods | por |
| dc.title | Evaluation of mini-VIDAS rapid test for detection of Listeria monocytogenes from production lines of fresh to cold-smoked fish | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| person.familyName | Vaz Velho | |
| person.familyName | Gibbs | |
| person.givenName | Manuela | |
| person.givenName | Paul | |
| person.identifier.ciencia-id | C719-383D-5548 | |
| person.identifier.orcid | 0000-0003-4825-2058 | |
| person.identifier.orcid | 0000-0002-6580-4363 | |
| person.identifier.rid | K-3058-2014 | |
| person.identifier.scopus-author-id | 55999738500 | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | ceca7b30-6960-4ad2-bb0c-675fcca89f86 | |
| relation.isAuthorOfPublication | 218d85c8-40f4-42c0-9a47-4e8ad9f72a96 | |
| relation.isAuthorOfPublication.latestForDiscovery | 218d85c8-40f4-42c0-9a47-4e8ad9f72a96 |