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Advisor(s)
Abstract(s)
This study was conducted to evaluate the efficacy of the mini-VIDAS Listeria monocytogenes (LMO) system (BioMe´rieux
Vitek, Inc., Missouri, USA) for detection of L. monocytogenes in environmental and fish samples from three Portuguese
cold-smoking plants and from their fresh fish suppliers. Mini-VIDAS-LMO is a fully automated system that uses fluorescent
ELFA (Enzyme Linked Fluorescent Assay) technology for detection of Listeria monocytogenes antigens in food. It can be a
rapid screening method alternative to time consuming classical isolation and identification. Two hundred and ninety five
samples were tested in mini-VIDAS-LMO and in parallel by the ISO 11290-1 traditional protocol. The mini-VIDAS-LMO
detected 8 of the 11 confirmed positive samples and presented 11 false positive results. The specificity of the mini-VIDASLMO
found in this experiment was 0.96 and the sensitivity 0.73
Description
Keywords
Cold-smoked fish Listeria monocytogenes Rapid immunological-based methods
Citation
VAZ-VELHO, Manuela; DUARTE, Gabriela; GIBBS, Paul - of Listeria monocytogenes from production lines of fresh to cold-smoked fish. Journal of Microbiological Methods. ISSN 0167-7012. Vol. 40 (2000), p. 147–151
Publisher
Elsevier