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Potential chitosan-coated alginate nanoparticles for ocular delivery of daptomycin

2015Journal article Restricted access
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dc.contributor.authorCosta, J. R.
dc.contributor.authorSilva, N. C.
dc.contributor.authorSarmento, B.
dc.contributor.authorPintado, M.
dc.date.accessioned2016-01-07T16:38:50Z
dc.date.available2016-01-07T16:38:50Z
dc.date.issued2015
dc.description.abstractDaptomycin may offer an antibacterial alternative for the treatment of endophthalmitis caused by methicillin-resistant Staphylococcus aureus (MRSA) and other potential agents. In the present project, mucoadhesive chitosan-coated alginate (CS-ALG) nanoparticles are proposed as an effective delivery system for daptomycin permeation across ocular epithelia, with potential for the treatment of bacterial endophthalmitis. CS-ALG nanoparticles were prepared by ionotropic pre-gelation of an alginate core followed by chitosan polyelectrolyte complexation, and characterized regarding particle size, polydispersity, and zeta potential. The encapsulation efficiency was determined and antimicrobial activity was also tested after encapsulation of the antibiotic. Also, in vitro ocular permeability of free daptomycin and encapsulation into chitosan and CS-ALG nanoparticles was evaluated using ocular epithelial cell culture models. Formulated daptomycin-loaded CS-ALG nanoparticles were negatively charged, with a size range of 380–420 nm, suitable for ocular application. The encapsulation efficiency was between 79 and 92 %, with decreasing alginate:daptomycin mass ratios. The antibacterial activity of daptomycin against major microorganisms responsible for bacterial endophthalmitis was not affected by encapsulation into nanoparticles. Daptomycin permeability was up to 16 % (chitosan nanoparticles) and 9 % (CS-ALG nanoparticles) through corneal cell monolayer, and 18 % (chitosan nanoparticles) and 12 % (CS-ALG nanoparticles) for retinal cell monolayer after 4 h, demonstrating epithelial retention of the drug compared to free drug. The developed daptomycin-loaded CS-ALG nanoparticles seem to be an interesting and potential system for ocular daptomycin delivery and treatment of bacterial endophthalmitis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationCOSTA, J. R.; SILVA, N. C.; SARMENTO, B.; PINTADO, M. E. - Potential chitosan-coated alginate nanoparticles for ocular delivery of daptomycin. European Journal of Clinical Microbiology & Infectious Diseases. ISSN 1435-4373. Vol. 34, n.º 6 (2015), p. 1255-1262pt_PT
dc.identifier.doi10.1007/s10096-015-2344-7pt_PT
dc.identifier.eid84939989660
dc.identifier.eissn0934-9723
dc.identifier.issn1435-4373
dc.identifier.pmid25754770
dc.identifier.urihttp://hdl.handle.net/10400.14/18853
dc.identifier.wos000354195100025
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer
dc.titlePotential chitosan-coated alginate nanoparticles for ocular delivery of daptomycinpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.citation.endPage1262
oaire.citation.issue6
oaire.citation.startPage1255
oaire.citation.titleEuropean Journal of Clinical Microbiology and Infectious Diseases
oaire.citation.volume34
oaire.fundingStream3599-PPCDT
person.familyNameRibeiro Costa
person.familyNameSilva
person.familyNameSarmento
person.familyNamePintado
person.givenNameJoana
person.givenNameNádia
person.givenNameBruno
person.givenNameMaria Manuela
person.identifier152609
person.identifier456608
person.identifier.ciencia-idD215-90DC-618A
person.identifier.ciencia-idE816-B6B0-06A5
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0003-0559-0540
person.identifier.orcid0000-0003-2090-4923
person.identifier.orcid0000-0001-5763-7553
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridV-9872-2017
person.identifier.ridJ-6265-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id41662079600
person.identifier.scopus-author-id57194334236
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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