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Early pathogen recognition and antioxidant system activation contributes to actinidia arguta tolerance against pseudomonas syringae pathovars actinidiae and actinidifoliorum

2020Journal article Open access
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dc.contributor.authorNunes da Silva, M.
dc.contributor.authorVasconcelos, M. W.
dc.contributor.authorGaspar, M.
dc.contributor.authorBalestra, G. M.
dc.contributor.authorMazzaglia, A.
dc.contributor.authorCarvalho, Susana M. P.
dc.date.accessioned2020-12-11T18:08:47Z
dc.date.available2020-12-11T18:08:47Z
dc.date.issued2020
dc.description.abstractActinidia chinensis and A. arguta have distinct tolerances to Pseudomonas syringae pv. actinidiae (Psa), but the reasons underlying the inter-specific variation remain unclear. This study aimed to integrate the metabolic and molecular responses of these two kiwifruit species against the highly pathogenic Psa and the less pathogenic P. syringae pv. actinidifoliorum (Pfm) bacterial strains. Disease development was monitored weekly till 21 days post inoculation (dpi), analysing a broad number and variety of parameters including: colony forming units (CFU), foliar symptoms, total chlorophylls, lipid peroxidation, soluble polyphenols, lignin and defense-related gene expression. At the end of the experimental period A. chinensis inoculated with Psa presented the highest endophytic bacterial population, whereas A. arguta inoculated with Pfm showed the lowest values, also resulting in a lower extent of leaf symptoms. Metabolic responses to infection were also more pronounced in A. chinensis with decreased total chlorophylls (up to 55%) and increased lipid peroxidation (up to 53%), compared with non-inoculated plants. Moreover, at 14 dpi soluble polyphenols and lignin concentrations were significantly higher (112 and 26%, respectively) in Psa-inoculated plants than in controls, while in A. arguta no significant changes were observed in those metabolic responses, except for lignin concentration which was, in general, significantly higher in Psa-inoculated plants (by at least 22%), comparing with control and Pfm-inoculated plants. Genes encoding antioxidant enzymes (SOD, APX and CAT) were upregulated at an earlier stage in Psa-inoculated A. arguta than in A. chinensis. In contrast, genes related with phenylpropanoids (LOX1) and ethylene (SAM) pathways were downregulated in A. arguta, but upregulated in A. chinensis in the later phases of infection. Expression of Pto3, responsible for pathogen recognition, occurred 2 dpi in A. arguta, but only 14 dpi in A. chinensis. In conclusion, we found that A. arguta is more tolerant to Psa and Pfm infection than A. chinensis and its primary and secondary metabolism is less impacted. A. arguta higher tolerance seems to be related with early pathogen recognition, the activation of plant antioxidant system, and to the suppression of ET and JA pathways from an earlier moment after infection.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, M.N., Vasconcelos, M.W., Gaspar, M., Balestra, G.M., Mazzaglia, A., Carvalho, S.M.P. (2020). Early pathogen recognition and antioxidant system activation contributes to actinidia arguta tolerance against pseudomonas syringae pathovars actinidiae and actinidifoliorum. Frontiers in Plant Science, 11pt_PT
dc.identifier.doi10.3389/fpls.2020.01022
dc.identifier.eid85089086493
dc.identifier.issn1664-462X
dc.identifier.pmcPMC7387506
dc.identifier.pmid32793252
dc.identifier.urihttp://hdl.handle.net/10400.14/31458
dc.identifier.wos000559191200001
dc.language.isoporpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontierspt_PT
dc.relationGreenUPorto - Research Center in Sustainable Agri-food Production
dc.relationGreenUPorto - Research Center in Sustainable Agri-food Production
dc.relationCentre for Biotechnology and Fine Chemistry
dc.relationKiwi Bacterial Canker: Exploring Tolerance Mechanisms and Novel Control Techniques
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectActinidia chinensispt_PT
dc.subjectAntioxidant systempt_PT
dc.subjectGene expressionpt_PT
dc.subjectPseudomonas syringae pv. actinidiaept_PT
dc.subjectPseudomonas syringe pv. actinidifoliorumpt_PT
dc.subjectSusceptibilitypt_PT
dc.titleEarly pathogen recognition and antioxidant system activation contributes to actinidia arguta tolerance against pseudomonas syringae pathovars actinidiae and actinidifoliorumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleGreenUPorto - Research Center in Sustainable Agri-food Production
oaire.awardTitleGreenUPorto - Research Center in Sustainable Agri-food Production
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardTitleKiwi Bacterial Canker: Exploring Tolerance Mechanisms and Novel Control Techniques
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-PRO%2F6156%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05748%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F05748%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F99853%2F2014/PT
oaire.citation.titleFrontiers in Plant Sciencept_PT
oaire.citation.volume11pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
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oaire.fundingStream6817 - DCRRNI ID
person.familyNameNunes da Silva
person.familyNameVasconcelos
person.familyNameMazzaglia
person.familyNameCarvalho
person.givenNameMarta
person.givenNameMarta
person.givenNameAngelo
person.givenNameSusana M.P.
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person.identifier.ridI-8166-2013
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project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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