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Bioaugmentation of Aerobic Granular Sludge with specialized degrading granules treating 2-fluorophenol wastewater

2019Conference object Open access
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dc.contributor.authorOliveira, Ana S.
dc.contributor.authorAmorim, Catarina L.
dc.contributor.authorZlopasa, Jure
dc.contributor.authorLin, Yumei
dc.contributor.authorLoosdrecht, Mark C. M. van
dc.contributor.authorCastro, Paula
dc.date.accessioned2020-02-18T19:57:30Z
dc.date.available2022-12-31T01:30:20Z
dc.date.issued2019
dc.description.abstractThe industry growth has been accompanied by an increase in the amount of industrial chemicals being released into the environment. Indigenous microbial communities in wastewater biotreatment processes are not always effective in removing xenobiotics. This work aimed to evaluate the efficiency of a new bioaugmentation strategy in an aerobic granular sludge sequencing batch reactor (AGS-SBR) system fed with 2-fluorophenol (2-FP). Bioreactor performance in terms of phosphate and ammonium removal, 2-FP degradation and chemical oxygen demand (COD) was evaluated. The new bioaugmentation strategy consisted in producing granules using extracellular polymeric substances (EPS) extracted from AGS as a carrying matrix and a 2-FP degrading strain, Rhodococcus sp. FP1. The produced granules were used for the bioaugmentation of a reactor fed with 2-FP. Shortly after bioaugmentation, the produced granules broke down into smaller fragments inside the bioreactor, but 2-FP degradation occurred. After 8 days of bioaugmentation, 2-FP concentration inside the reactor started to decrease, and stoichiometric fluorine release was observed 35 days later. Phosphate and ammonium removal also improved after bioaugmentation, increasing from 30% to 38% and from 20 to 27%, respectively. Complete ammonium removal was only achieved when 2-FP feeding stopped, and phosphate removal was not recovered during operation time. COD removal also improved after the addition of the produced granules. The persistence of Rhodococcus sp. FP1 in the reactor was followed by qPCR. Rhodococcus sp. FP1 was detected 1 day after in the AGS and up to 3 days after bioaugmentation at the effluent. Nevertheless, the 2-FP degradative ability remained thereafter in the granules. Horizontal gene transfer could have happened from the 2-FP degrading strain to indigenous microbiome as some bacteria isolated from the AGS, 3 months after bioaugmentation, were able to degrade 2-FP. This study presents a promising and feasible bioaugmentation strategy to introduce specialized bacteria into AGS systems treating recalcitrant pollutants in wastewater.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.14/29609
dc.language.isoengpt_PT
dc.relationPOCI-01-0145-FEDER-031264
dc.relationCentre for Biotechnology and Fine Chemistry
dc.relationAGeNT - Aerobic Granular Sludge technology combined with selected microbial agents to degrade toxic pollutants from wastewater
dc.subjectAerobic granular sludgept_PT
dc.subjectBioaugmentationpt_PT
dc.subjectBioreactorpt_PT
dc.subject2-fluorophenolpt_PT
dc.titleBioaugmentation of Aerobic Granular Sludge with specialized degrading granules treating 2-fluorophenol wastewaterpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardTitleAGeNT - Aerobic Granular Sludge technology combined with selected microbial agents to degrade toxic pollutants from wastewater
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBTA-BTA%2F31264%2F2017/PT
oaire.citation.conferencePlacePortugalpt_PT
oaire.citation.titleMicrobiotec 19pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
person.familyNameOliveira
person.familyNameAmorim
person.familyNamevan Loosdrecht
person.familyNameCastro
person.givenNameAna S.
person.givenNameCatarina L.
person.givenNameMark
person.givenNamePaula
person.identifier1394766
person.identifier2013444
person.identifier.ciencia-idF31D-DEA6-9783
person.identifier.ciencia-id791E-5D42-9B68
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0002-3070-1604
person.identifier.orcid0000-0002-6756-552X
person.identifier.orcid0000-0003-0658-4775
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridK-6091-2014
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id55532795700
person.identifier.scopus-author-id36896692500
person.identifier.scopus-author-id7004463801
person.identifier.scopus-author-id7102781782
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctOs resultados apresentados ainda não se encontram publicados em revista cientifica, nem em tese de doutoramento.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication04a1cc1a-24db-4ebd-8ca4-72c8f729c586
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relation.isAuthorOfPublicationf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isAuthorOfPublication.latestForDiscovery82d7f5ef-4aea-41c3-b2eb-29e19264b0af
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relation.isProjectOfPublication.latestForDiscovery1f76a7db-2520-482f-b56b-7e01ea36dd1f

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