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Florfenicol (FF) is a synthetic antibiotic, widely used in veterinary medicine. It is one of the few approved antibiotics for use in aquaculture during both production and processing operations, mainly to prevent and treat bacterial diseases. Therefore, this antibiotic is inevitably presented in the effluents from fish farms and eventually directly discharged in surface waters. Removal of FF has been mainly reported using physical-chemical processes but reports on its removal by biological processes is scarce. Bioremediation has been considered the strategy of choice to reduce the risk of hazardous chemicals in the environment. Therefore, microorganisms able to tolerate and degrade FF could represent added value towards more efficient biological removal processes. The main aim of this study was to investigate FF degradation by the bacterial strain Labrys portucalensis F11, capable to degrade other pharmaceuticals.