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Release of polyphenols from carnauba wax nanoparticles

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dc.contributor.authorCampos, Débora
dc.contributor.authorMadureira, Raquel
dc.contributor.authorGomes, Ana M. P.
dc.contributor.authorSarmento, Bruno
dc.contributor.authorPintado, Maria Manuela
dc.date.accessioned2014-05-08T19:00:24Z
dc.date.available2014-05-08T19:00:24Z
dc.date.issued2013
dc.description.abstractThe inclusion in foods of compounds with antioxidant activity from rich sources (fruits, aromatic herbs, etc.) has become a common procedure of the food industry. However, when incorporated in food matrix these compounds may interact with matrix components and reduce or loss bioactivity. Hence, the formulation of loaded polyphenols nanoparticles may offer a way to protect such compounds against degradation. In this work, the releasing rates from Solid Lipid Nanoparticles (SLNs) of rosmarinic acid (RA) were tested. Moreover, the effect of selected SLNs upon DNA i.e. antioxidant and prooxidant effects were also evaluated. Different formulations of SLNs were tested, using 0.5% (w/v) of carnauba wax and 1 and 2% (v/v) of polysorbate 80, prepared by a hot melt ultrasonication method. Two types of in vitro release tests were performed. The first approach used a cellulose acetate dialysis bag with molecular weight cut-off of 12 kDa, to evaluate the direct release of RA from the SLNs. The experiment was conducted during 12 h, at 37 ± 0.5 °C with continuous homogenization. The SLNs were dispersed in PBS (0.1 M, pH 7.4), and samples were withdrawn at different time points during the experiment (0, 1, 2, 4, 6, 8, 10 and 12 h). The polyphenol content of samples was analysed by HPLC. The second test using intestinal-based cell co-culture (Caco-2 and HT29-MTX cells), was performed to study the cells permeability to the RA and the possible presence of secondary metabolites from RA. The passage through the cell monolayer and cytotoxicity were evaluated by HPLC and TEER monitoring, respectively. The SLNs were diluted in sterilized ultrapure water, the solution was placed in the apical zone of the cells and samples were taken from the sub-apical zone, at different times. In both in vitro release tests the results have shown high % of RA release. The test using dialysis bag showed 50% RA release after 4h of experiment, and by 12h the % of release reached ca. 90%. On the other side, tests using intestinal cell lines showed that the Caco-2 cells have higher % of passage of RA (ca. 60%) for the sub-apical zone, when compared with the set of Caco-2 and HT29-MTX cells. Finally, SLNs do not attack DNA chains, but also do not protect the DNA, demonstrating that SLNs have a neutral effect, whereas free RA demonstrated a protective effect on the DNA chain.
dc.identifier.citationCAMPOS, Débora ...[et al.] - Release of polyphenols from carnauba wax nanoparticles. In Portuguese Congress of Microbiology and Biotechnology' 2013, Aveiro, Portugal, 6-8 December 2013 – In MICROBIOTECH 2013: Book of Abstracts. p. 481. Available at: http://microbiotec13.web.ua.pt/docs/e-book%20Microbiotec%202013%20Aveiro.pdfpor
dc.identifier.urihttp://hdl.handle.net/10400.14/14372
dc.language.isoengpor
dc.titleRelease of polyphenols from carnauba wax nanoparticlespor
dc.typeconference object
dspace.entity.typePublication
person.familyNameMadureira
person.familyNameGomes
person.familyNamePintado
person.givenNameRaquel
person.givenNameAna Maria
person.givenNameMaria Manuela
person.identifierM-4659-2013
person.identifier162628
person.identifier456608
person.identifier.ciencia-id1C14-25F8-CC07
person.identifier.ciencia-idAA12-BFC1-B2E3
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0001-6596-1927
person.identifier.orcid0000-0001-7883-2446
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridM-4659-2013
person.identifier.ridB-9944-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id8384097900
person.identifier.scopus-author-id57190668768
person.identifier.scopus-author-id7004483898
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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relation.isAuthorOfPublicationc18c2fb1-f3dc-4274-a646-23d456432798
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relation.isAuthorOfPublication.latestForDiscoveryba387c7d-27c9-4016-895c-b35597e91ebc

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