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Use of response surface methodology to optimize protease synthesis by a novel strain of Bacillus sp. isolated from Portuguese sheep wool

2012Journal article Restricted access
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dc.contributor.authorQueiroga, A.C.
dc.contributor.authorPintado, M.E.
dc.contributor.authorMalcata, F.X.
dc.date.accessioned2013-01-28T14:13:40Z
dc.date.available2013-01-28T14:13:40Z
dc.date.issued2012
dc.description.abstractAims: To investigate the influence of yeast extract, peptone, temperature and pH upon protease productivity by Bacillus sp. HTS102 – a novel wild strain isolated from wool of a Portuguese sheep breed (Merino). Methods and Results: A 24 full factorial, central composite design together with response surface methodology was used to carry out the experiments and analyse the results, respectively. Among the individual parameters tested, temperature and peptone concentration produced significant effects upon protease productivity. A high correlation coefficient (R2 = 0Æ994, P < 0Æ01) indicated that the empiric second-order polynomial model postulated was adequate to predict said productivity, with the optimum loci characterized by: temperature of 43 C, peptone content of 1Æ4 g l)1, pH of 5Æ1 and yeast extract concentration of 10Æ0 g l)1. Conclusions: Protease synthesis depends chiefly on temperature and peptone level. The maximum protease activity was more than twice that obtained with the basal medium, so the experimental design and analysis undertaken were effective towards process optimization. Significance and Impact of the Study: Rational choice of processing conditions for maximum protease productivity will be relevant if an economically feasible fermentation process based on Bacillus sp. HTS102 is intended.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationQUEIROGA, A. C.; PINTADO, M. E.; MALCATA, F. Xavier - Use of response surface methodology to optimize protease synthesis by a novel strain of Bacillus sp. isolated from Portuguese sheep wool. Journal of Applied Microbiology. ISSN 1365-2672. Vol. 113, n.º 1 (2012), p. 36–43por
dc.identifier.doi10.1111/j.1365-2672.2012.05300.x
dc.identifier.eissn1365-2672
dc.identifier.issn1364-5072
dc.identifier.urihttp://hdl.handle.net/10400.14/10001
dc.identifier.wosWOS:000305184900004
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherThe Society for Applied Microbiologypor
dc.subjectProteolytic activitypor
dc.subjectSheep woolpor
dc.subjectThermotolerant enzymepor
dc.subjectWild bacteriapor
dc.titleUse of response surface methodology to optimize protease synthesis by a novel strain of Bacillus sp. isolated from Portuguese sheep woolpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F19121%2F2004/PT
oaire.citation.endPage43
oaire.citation.startPage36
oaire.citation.titleJournal of Applied Microbiology
oaire.citation.volume113
oaire.fundingStreamSFRH
person.familyNameQueiroga
person.familyNamePintado
person.familyNameMalcata
person.givenNameAna Catarina
person.givenNameMaria Manuela
person.givenNameFrancisco
person.identifier456608
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0001-8395-1837
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridJ-7815-2012
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id16067735500
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id7102542478
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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