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Quinolone resistant Aeromonas spp. as carriers and potential tracers of acquired antibiotic resistance in hospital and municipal wastewater

2016Journal article Restricted access
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dc.contributor.authorVarela, Ana Rita
dc.contributor.authorNunes, Olga C.
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2016-04-21T14:38:37Z
dc.date.available2016-04-21T14:38:37Z
dc.date.issued2016
dc.description.abstractMembers of the genus Aeromonas are recognized carriers of antibiotic resistance in aquatic environments. However, their importance on the spread of resistance from hospital effluents to the environment is poorly understood. Quinolone resistant Aeromonas spp. (n = 112) isolated from hospital effluent (HE) and from raw (RWW) and treated wastewater (TWW) of the receiving urban wastewater treatment plant (UWTP) were characterized. Species identification and genetic intraspecies diversity were assessed based on the 16S rRNA, cpn60 and gyrB genes sequence analysis. The antibiotic resistance phenotypes and genotypes (qnrA, qnrB, qnrC, qnrD, qnrS, qnrVC; qepA; oqxAB; aac(6′)-Ib-cr; blaOXA; incU) were analyzed in function of the origin and taxonomic group. Most isolates belonged to the species Aeromonas caviae and Aeromonas hydrophila (50% and 41%, respectively). The quinolone and the beta-lactamase resistance genes aac(6′)-Ib-cr and blaOXA, including gene blaOXA-101, identified for the first time in Aeromonas spp., were detected in 58% and 56% of the isolates, respectively, with identical prevalence in HE and UWTP wastewater. In contrast, the gene qnrS2was observedmainly in isolates from the UWTP (51%) and rarely in HE isolates (3%), suggesting that its origin is not the clinical setting. Bacterial groups and genes that allowthe identification of major routes of antibiotic resistance dissemination are valuable tools to control this problem. In this study, itwas concluded that members of the genus Aeromonas harboring the genes aac(6′)-Ib-cr and blaOXA are relevant tracers of antibiotic resistance dissemination inwastewater habitats, while those yielding the gene qnrS2 allow the traceability from non-clinical sources.pt_PT
dc.identifier.citationVARELA, Ana Rita; NUNES, Olga C.; MANAIA, C. M. - Quinolone resistant Aeromonas spp. as carriers and potential tracers of acquired antibiotic resistance in hospital and municipal wastewater. Science of the Total Environment. ISSN 0048-9697. Vol. 542 (2016), p. 665–671pt_PT
dc.identifier.eid84946780842
dc.identifier.pmid26546762
dc.identifier.urihttp://hdl.handle.net/10400.14/19904
dc.identifier.wos000365602100067
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.relationDYNAMICS OF ANTIMICROBIAL RESISTANCE BETWEEN THE HOSPITAL AND THE ENVIRONMENT
dc.relationTRACKING ANTIBIOTIC RESISTANCE FROM HOSPITAL EFFLUENTS TO THE SURROUNDING ENVIRONMENT
dc.subjectAeromonaspt_PT
dc.subjectQuinolone resistancept_PT
dc.subjectHospital effluentpt_PT
dc.titleQuinolone resistant Aeromonas spp. as carriers and potential tracers of acquired antibiotic resistance in hospital and municipal wastewaterpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDYNAMICS OF ANTIMICROBIAL RESISTANCE BETWEEN THE HOSPITAL AND THE ENVIRONMENT
oaire.awardTitleTRACKING ANTIBIOTIC RESISTANCE FROM HOSPITAL EFFLUENTS TO THE SURROUNDING ENVIRONMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAC-AMB%2F113840%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F70986%2F2010/PT
oaire.citation.endPage671
oaire.citation.startPage665
oaire.citation.titleScience of the Total Environment
oaire.citation.volume542
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameVarela
person.familyNameNunes
person.familyNameManaia
person.givenNameAna Rita
person.givenNameOlga
person.givenNameCélia
person.identifier.ciencia-idBA14-BD30-C9AF
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0001-9691-0217
person.identifier.orcid0000-0003-4742-2537
person.identifier.orcid0000-0002-3674-1789
person.identifier.scopus-author-id7005088596
person.identifier.scopus-author-id6602465318
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isProjectOfPublication00130e45-522a-49ee-9fb0-3c4605578317
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