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Advisor(s)
Abstract(s)
Members of the genus Aeromonas are recognized carriers of antibiotic resistance in aquatic environments. However,
their importance on the spread of resistance from hospital effluents to the environment is poorly understood. Quinolone
resistant Aeromonas spp. (n = 112) isolated from hospital effluent (HE) and from raw (RWW) and treated
wastewater (TWW) of the receiving urban wastewater treatment plant (UWTP) were characterized. Species identification
and genetic intraspecies diversity were assessed based on the 16S rRNA, cpn60 and gyrB genes sequence
analysis. The antibiotic resistance phenotypes and genotypes (qnrA, qnrB, qnrC, qnrD, qnrS, qnrVC; qepA; oqxAB;
aac(6′)-Ib-cr; blaOXA; incU) were analyzed in function of the origin and taxonomic group. Most isolates belonged
to the species Aeromonas caviae and Aeromonas hydrophila (50% and 41%, respectively). The quinolone and the
beta-lactamase resistance genes aac(6′)-Ib-cr and blaOXA, including gene blaOXA-101, identified for the first time in
Aeromonas spp., were detected in 58% and 56% of the isolates, respectively, with identical prevalence in HE and
UWTP wastewater. In contrast, the gene qnrS2was observedmainly in isolates from the UWTP (51%) and rarely
in HE isolates (3%), suggesting that its origin is not the clinical setting.
Bacterial groups and genes that allowthe identification of major routes of antibiotic resistance dissemination are
valuable tools to control this problem. In this study, itwas concluded that members of the genus Aeromonas harboring
the genes aac(6′)-Ib-cr and blaOXA are relevant tracers of antibiotic resistance dissemination inwastewater
habitats, while those yielding the gene qnrS2 allow the traceability from non-clinical sources.
Description
Keywords
Aeromonas Quinolone resistance Hospital effluent
Citation
VARELA, Ana Rita; NUNES, Olga C.; MANAIA, C. M. - Quinolone resistant Aeromonas spp. as carriers and potential tracers of acquired antibiotic resistance in hospital and municipal wastewater. Science of the Total Environment. ISSN 0048-9697. Vol. 542 (2016), p. 665–671
Publisher
Elsevier