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Aerobic granular sludge has EPS-producing bacteria able to tolerate salt

2019-12-05Documento de conferência Acesso aberto
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dc.contributor.authorPaulo, Ana M. S.
dc.contributor.authorAmorim, Catarina L.
dc.contributor.authorCastro, Paula M. L.
dc.date.accessioned2020-03-02T16:25:08Z
dc.date.available2020-03-02T16:25:08Z
dc.date.issued2019-12-05
dc.description.abstractThe aerobic granular sludge (AGS) process is a promising biotechnology which relies on the formation of compact biomass granules. Granulation occurs due to the overproduction of extracellular polymeric substances (EPS) by some microbes in response to stress conditions. EPS protect bacteria from the effect of toxic or inhibiting compounds present in the wastewater, such as salts. One of the current challenges is to use the AGS process to treat high salinity wastewater, commonly produced by agro-food and chemical industries. The main objective of this study was to screen for EPS-producing bacteria bacteria in an AGS reactor treating synthetic saline wastewater contaminated with a toxic compound. Several bacterial isolates were obtained from the reactor biomass. Genomic DNA was extracted and isolates (30) were grouped according to species similarity, based on RAPD profiles. Isolates displaying unique profiles (15) were subsequently identified by 16S rRNA gene sequencing analysis. Bacteria highly related to Pseudomonas, Aeromonas, Stenotrophomonas, Flavobacterium and Pseudoxanthomonas were obtained. Isolates SG4 (Stenotrophomonas) and FG10 (Flavobacterium) belong to bacterial genera associated to EPS production in granules. These were selected for growth and biofilm formation assays with increasing NaCl concentrations (0 to 35 g L-1). Both isolates were able to grow in the presence of 35 g NaCl L-1, despite at a lower growth rate. Although salt increase affected biofilm production, SG4 was the best biofilm producer. EPS production by SG4 in the presence of 10 and 20 g L-1 of NaCl was compared. EPS was extracted and the content in proteins, humic acids and carbohydrates was quantified. SG4 was able to produce more EPS in the presence of 10 g L-1 (123 mg g-1 VSS) compared to 20 g L-1 of NaCl (77.6 mg g-1 VSS). EPS-producing bacteria with ability to tolerate high salinity were retrieved from an AGS process treating synthetic wastewater. Further research is required to gain more knowledge on these bacteria and their importance for the robustness of a process treating saline wastewater.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPaulo, A. M. S., Amorim, C. L., Castro, P. M. L. (2019). Aerobic granular sludge has EPS-producing bacteria able to tolerate salt. In [Abstract] MicroBiotec: Congress Of Microbiology And Biotechnology 2019, December 5th-7th, Coimbra, Portugal. (p. 122). Coimbra: University (Pólo II)pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.14/29753
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherUniversidade de Coimbrapt_PT
dc.subjectExtracellular Polymeric Substances (EPS)pt_PT
dc.subjectHalotolerant bacteriapt_PT
dc.subjectAerobic Granular Sludgept_PT
dc.subjectHigh salinitypt_PT
dc.titleAerobic granular sludge has EPS-producing bacteria able to tolerate saltpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceCoimbrapt_PT
oaire.citation.startPage122pt_PT
oaire.citation.titleMicrobiotec 2019 - Congress of Microbiology and Biotechnologypt_PT
person.familyNameAmorim
person.familyNameCastro
person.givenNameCatarina L.
person.givenNamePaula
person.identifier1394766
person.identifier2013444
person.identifier.ciencia-id791E-5D42-9B68
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0002-6756-552X
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridK-6091-2014
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id36896692500
person.identifier.scopus-author-id7102781782
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication17a25a6a-22d5-40bc-9183-0c81b79a89bf
relation.isAuthorOfPublicationf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isAuthorOfPublication.latestForDiscovery17a25a6a-22d5-40bc-9183-0c81b79a89bf

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