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Hydrogen sulfide biological oxidation by pure cultures of heterotrophic bacteria

2014Conference object Open access
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dc.contributor.authorNovo, Ana
dc.contributor.authorAntunes, Filipa
dc.contributor.authorBaptista, Inês
dc.contributor.authorJorge, Ruben
dc.contributor.authorSaraiva, Isabel
dc.contributor.authorMoreira, Irina S.
dc.contributor.authorCastro, Paula M. L.
dc.date.accessioned2015-10-21T10:49:07Z
dc.date.available2015-10-21T10:49:07Z
dc.date.issued2014
dc.description.abstractBiogas is a mixture of gases produced during anaerobic treatment of sludge whose composition depends on the type of digested materials as well as on the operating conditions of bioreactor. This consists mainly of CH4 and CO2, but components such as H2S is of particular interest due to its corrosive, toxic and environmentally hazardous properties. Removal of H2S present in biogas can be achieved through physical and chemical processes, which are effective but produce secondary waste, which in turn gives rise to another problem of pollution. Biological treatment can be used to removing H2S using different species of microorganisms whose specific enzymes catalyze the biological oxidation of H2S, including photoautotrophic and chemotrophs organisms. This work aims to compare the biotechnological removal of H2S from biogas in aerobic conditions through heterotrophic microbial biomass and biocatalytic treatments. Heterotrophic microorganisms were isolated from microbial enrichments supplied with H2S streams from a wastewater treatment plant and were characterized based on their ability to grow in mineral medium with acetate as source of carbon. Isolates retrieved were mainly affiliated to the class of γ-proteobacteria with a strong prevalence of the genus Pseudomonas. Isolates A9, B9 and C1 all identified as Pseudomonas spp., revealed as the species with higher potential for H2S removal, growing at concentrations of H2S up to 16 mM. Total protein profiles were screened by SDS-PAGE for the three isolates, before and after the addition of H2S, with the aim of purifying enzyme fractions involved in the oxidation process.pt_PT
dc.identifier.citationNOVO, Ana [et al.] - Hydrogen sulfide biological oxidation by pure cultures of heterotrophic bacteria . In XVI International Biodeterioration and Biodegradation Symposium, Lodz, Poland, 3-5 September, 2014 [abstract]pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.14/18511
dc.language.isoengpt_PT
dc.relationQREN-ADI 21592pt_PT
dc.relationStrategic Project - LA 16 - 2013-2014
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHydrogen sulfidept_PT
dc.subjectbiological oxidationpt_PT
dc.subjectPseudomonas sp.pt_PT
dc.subjectsulfide oxidasept_PT
dc.titleHydrogen sulfide biological oxidation by pure cultures of heterotrophic bacteriapt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 16 - 2013-2014
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameNovo
person.familyNameAntunes
person.familyNameMoreira
person.familyNameCastro
person.givenNameAna
person.givenNameFilipa
person.givenNameIrina Susana Sousa
person.givenNamePaula
person.identifierK-5841-2014
person.identifier2013444
person.identifier.ciencia-id8C1B-682F-0E52
person.identifier.ciencia-id161E-A350-7FDE
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0001-8288-2825
person.identifier.orcid0000-0002-8472-078X
person.identifier.orcid0000-0001-6516-0994
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridK-5841-2014
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id57220480925
person.identifier.scopus-author-id36798094100
person.identifier.scopus-author-id7102781782
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationed9ebe97-8eab-4078-93e0-28f13a4848d7
relation.isAuthorOfPublication8c1324e1-e031-4818-8c34-dcb487114b41
relation.isAuthorOfPublicationbb01f335-e184-4cb9-ae8a-70e62fdd5df3
relation.isAuthorOfPublicationf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isAuthorOfPublication.latestForDiscoverybb01f335-e184-4cb9-ae8a-70e62fdd5df3
relation.isProjectOfPublication286113dc-d170-44f7-afcc-cbb22f81f7b3
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