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HPLC-FD Method Validation for Determination of Several Fluoroquinolones in Biodegradation Assays

dc.contributor.authorMaia, Alexandra S.
dc.contributor.authorRibeiro, Ana R.
dc.contributor.authorCastro, Paula M. L.
dc.contributor.authorTiritan, Maria E.
dc.date.accessioned2013-11-14T16:40:03Z
dc.date.available2013-11-14T16:40:03Z
dc.date.issued2011-06
dc.description.abstractThe present concern in pharmaceuticals in the environment is well known and research studies in this area have been regularly reported [1]. Pharmaceuticals reach the environment by several ways but mostly due to their incorrect disposal and the incomplete elimination during the treatment processes in Wastewater Treatment Plants (WWTP). These residues continuously enter aquatic environments and many of them are resistant to degradation, being so called as pseudo-persistent pollutants [2]. In aquatic compartments, pharmaceutical residues reach concentrations in the ng/L to µg/L range [3]. Antibiotics are a class extensively studied due to its implications on development of multi-resistant bacteria. This work describes a validated HPLC (High Performance Liquid Chromatography) method with Fluorescence Detection for monitorization of biodegradation of four fluoroquinolones: Ofloxacin (OFL), Norfloxacin (NOR), Ciprofloxacin (CPF) and Moxifloxacin (MOX). The separation of the target pharmaceuticals was performed at 38ºC using a Luna 3µ PFP(2) 100 A (150 x 4.60 mm) column in isocratic mode with a mixture of 0.1% triethylamine (TEA) in water acidified to pH = 2.2 with trifluoroacetic acid (TFA) and ethanol as mobile phase. The validated method demonstrated selectivity, linearity (r2>0.999) and precision (intra-day: 0.71>RSD>2.51; inter-day: 1.11>RSD>2.74) in the range of 0.5 µg/mL – 12 µg/mL. The quantification limits were 5 ng/mL for OFL, NOR and CPF and 20 ng/mL for MOX. The biodegradation study was performed during 15 days using a microbial consortia constituted by three bacterial species known to degrade fluorinated compounds. The OFL presented higher extent of degradation than CPF and MOX.por
dc.identifier.citationMAIA, Alexandra [et al.] - HPLC-FD Method Validation for Determination of Several Fluoroquinolones in Biodegradation Assays. In 36th International Symposium on High-Performance Liquid Phase Separations and Related Techniques (HPLC 2011), Budapest, Hungary, 19–23 June 2011por
dc.identifier.urihttp://hdl.handle.net/10400.14/13244
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectbiodegradationpor
dc.subjectfluoroquinolonespor
dc.titleHPLC-FD Method Validation for Determination of Several Fluoroquinolones in Biodegradation Assayspor
dc.typeconference object
dspace.entity.typePublication
person.familyNameMaia
person.familyNameCastro
person.familyNameTiritan
person.givenNameAlexandra S.
person.givenNamePaula
person.givenNameMaria Elizabeth
person.identifier2013444
person.identifier1310409
person.identifier.ciencia-id6A15-F0A3-AEF2
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.orcid0000-0002-4167-383X
person.identifier.orcid0000-0001-8841-6606
person.identifier.orcid0000-0003-3320-730X
person.identifier.ridM-8241-2013
person.identifier.ridK-9492-2014
person.identifier.scopus-author-id55332349300
person.identifier.scopus-author-id7102781782
person.identifier.scopus-author-id6602890981
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublication56eedbbf-8105-4d55-bf1f-e38c62ed4da5
relation.isAuthorOfPublicationf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isAuthorOfPublication1ab8e8f5-cf82-4edd-89d8-46e5cca13e19
relation.isAuthorOfPublication.latestForDiscovery1ab8e8f5-cf82-4edd-89d8-46e5cca13e19

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