Repository logo
 
Publication

Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol

2012Journal article Restricted access
Simple item page
dc.contributor.authorDuque, Anouk F.
dc.contributor.authorHasan, Syed A.
dc.contributor.authorBessa, Vânia S.
dc.contributor.authorCarvalho, Maria F.
dc.contributor.authorSamin, Ghufrana
dc.contributor.authorJanssen, Dick B.
dc.contributor.authorCastro, Paula M. L.
dc.date.accessioned2012-01-16T17:11:27Z
dc.date.available2012-01-16T17:11:27Z
dc.date.issued2012
dc.description.abstractA pure bacterial culture able to utilize 2- fluorophenol (2-FP) as sole carbon and energy source was isolated by selective enrichment from sediments collected from a contaminated site in Northern Portugal. 16S rRNA gene analysis showed that the organism (strain FP1) belongs to the genus Rhodococcus. When grown aerobically on 2-FP, growth kinetics of strain FP1 followed the Luong model. An inhibitory effect of increasing 2-FP concentrations was observed with no growth occurring at 2- FP levels higher than ca. 4 mM. Rhodococcus strain FP1 was able to degrade a range of other organofluorine compounds, including 2-fluorobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-fluorophenol, 4-fluorophenol, 3-fluorocatechol, and 4-fluorocatechol, as well as chlorinated compounds such as 2-chlorophenol and 4-chlorophenol. Experiments with cell-free extracts and partially purified enzymes indicated that the first step of 2-fluorophenol metabolism was conversion to 3-fluorocatechol, suggesting an unusual pathway for fluoroaromatic metabolism. To our knowledge, this is the first time that utilization of 2-FP as a growth substrate by a pure bacterial culture is reported.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationDUQUE, Anouk F... [et al.] (2012) - Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol. Applied Microbiology and Biotechnology. ISSN 0175-7598. Vol.95, nº2 (2012) p.511-520por
dc.identifier.doi10.1007/s00253-011-3696-2
dc.identifier.eid84864666633
dc.identifier.eissn1432-0614
dc.identifier.issn0175-7598
dc.identifier.pmid22101783
dc.identifier.urihttp://hdl.handle.net/10400.14/7480
dc.identifier.wos000305227100022
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relationBIOLOGICAL PRODUCTION OF INDUSTRIALLY RELEVANT FLUOROORGANIC COMPOUNDS
dc.relationBIOCAT - biocatalytic process for the "green" production of value-added fluoroaromatic comppounds
dc.subject2-Fluorophenol (2-FP)por
dc.subjectRhodococcus sp.por
dc.subjectBiodegradationpor
dc.subjectLuong modelpor
dc.subjectMonooxygenasepor
dc.titleIsolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenolpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBIOLOGICAL PRODUCTION OF INDUSTRIALLY RELEVANT FLUOROORGANIC COMPOUNDS
oaire.awardTitleBIOCAT - biocatalytic process for the "green" production of value-added fluoroaromatic comppounds
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F30771%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F44670%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FV.5%2FA0105%2F2005/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F67306%2F2006/PT
oaire.citation.endPage520
oaire.citation.issue2
oaire.citation.startPage511
oaire.citation.titleApplied Microbiology and Biotechnology
oaire.citation.volume95
oaire.fundingStreamSFRH
oaire.fundingStreamPOCI
oaire.fundingStream3599-PPCDT
person.familyNameF. Duque
person.familyNameBessa
person.familyNameCarvalho
person.familyNameCastro
person.givenNameAnouk
person.givenNameVânia
person.givenNameM. Fátima
person.givenNamePaula
person.identifier2013444
person.identifier.ciencia-id7819-FA0D-FC04
person.identifier.ciencia-id6913-1464-155B
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0002-8360-1404
person.identifier.orcid0000-0002-2676-7184
person.identifier.orcid0000-0002-7181-0540
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridN-5863-2014
person.identifier.ridK-8029-2014
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id36573459300
person.identifier.scopus-author-id35214245200
person.identifier.scopus-author-id14038677200
person.identifier.scopus-author-id7102781782
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationd23f1d0d-9558-4f84-aa79-1efc0552f360
relation.isAuthorOfPublication3b8f352a-b4a7-4b65-bf31-3e975a7195bc
relation.isAuthorOfPublication964897d5-02c3-4efe-a8c3-44ea68cf4974
relation.isAuthorOfPublicationf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isAuthorOfPublication.latestForDiscovery3b8f352a-b4a7-4b65-bf31-3e975a7195bc
relation.isProjectOfPublication134016d3-93f5-44dc-a777-abc960720254
relation.isProjectOfPublication9db40366-da5f-4236-8a16-8bd9bd05325c
relation.isProjectOfPublication663222d6-a2ef-4983-b514-4b61ccd6b660
relation.isProjectOfPublication7a6da1f5-535e-4038-8b69-40b6da582063
relation.isProjectOfPublication.latestForDiscovery7a6da1f5-535e-4038-8b69-40b6da582063

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
19820448.pdf
Size:
277.96 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.44 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CBQF - Contribuições em Revistas Científicas / Contribution to Journals