Publication
Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol
dc.contributor.author | Duque, Anouk F. | |
dc.contributor.author | Hasan, Syed A. | |
dc.contributor.author | Bessa, Vânia S. | |
dc.contributor.author | Carvalho, Maria F. | |
dc.contributor.author | Samin, Ghufrana | |
dc.contributor.author | Janssen, Dick B. | |
dc.contributor.author | Castro, Paula M. L. | |
dc.date.accessioned | 2012-01-16T17:11:27Z | |
dc.date.available | 2012-01-16T17:11:27Z | |
dc.date.issued | 2012 | |
dc.description.abstract | A pure bacterial culture able to utilize 2- fluorophenol (2-FP) as sole carbon and energy source was isolated by selective enrichment from sediments collected from a contaminated site in Northern Portugal. 16S rRNA gene analysis showed that the organism (strain FP1) belongs to the genus Rhodococcus. When grown aerobically on 2-FP, growth kinetics of strain FP1 followed the Luong model. An inhibitory effect of increasing 2-FP concentrations was observed with no growth occurring at 2- FP levels higher than ca. 4 mM. Rhodococcus strain FP1 was able to degrade a range of other organofluorine compounds, including 2-fluorobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-fluorophenol, 4-fluorophenol, 3-fluorocatechol, and 4-fluorocatechol, as well as chlorinated compounds such as 2-chlorophenol and 4-chlorophenol. Experiments with cell-free extracts and partially purified enzymes indicated that the first step of 2-fluorophenol metabolism was conversion to 3-fluorocatechol, suggesting an unusual pathway for fluoroaromatic metabolism. To our knowledge, this is the first time that utilization of 2-FP as a growth substrate by a pure bacterial culture is reported. | por |
dc.description.version | info:eu-repo/semantics/publishedVersion | |
dc.identifier.citation | DUQUE, Anouk F... [et al.] (2012) - Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol. Applied Microbiology and Biotechnology. ISSN 0175-7598. Vol.95, nº2 (2012) p.511-520 | por |
dc.identifier.doi | 10.1007/s00253-011-3696-2 | |
dc.identifier.eid | 84864666633 | |
dc.identifier.eissn | 1432-0614 | |
dc.identifier.issn | 0175-7598 | |
dc.identifier.pmid | 22101783 | |
dc.identifier.uri | http://hdl.handle.net/10400.14/7480 | |
dc.identifier.wos | 000305227100022 | |
dc.language.iso | eng | por |
dc.peerreviewed | yes | por |
dc.publisher | Springer | por |
dc.relation | BIOLOGICAL PRODUCTION OF INDUSTRIALLY RELEVANT FLUOROORGANIC COMPOUNDS | |
dc.relation | BIOCAT - biocatalytic process for the "green" production of value-added fluoroaromatic comppounds | |
dc.subject | 2-Fluorophenol (2-FP) | por |
dc.subject | Rhodococcus sp. | por |
dc.subject | Biodegradation | por |
dc.subject | Luong model | por |
dc.subject | Monooxygenase | por |
dc.title | Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol | por |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.awardTitle | BIOLOGICAL PRODUCTION OF INDUSTRIALLY RELEVANT FLUOROORGANIC COMPOUNDS | |
oaire.awardTitle | BIOCAT - biocatalytic process for the "green" production of value-added fluoroaromatic comppounds | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F30771%2F2006/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F44670%2F2008/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/POCI/POCI%2FV.5%2FA0105%2F2005/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F67306%2F2006/PT | |
oaire.citation.endPage | 520 | |
oaire.citation.issue | 2 | |
oaire.citation.startPage | 511 | |
oaire.citation.title | Applied Microbiology and Biotechnology | |
oaire.citation.volume | 95 | |
oaire.fundingStream | SFRH | |
oaire.fundingStream | POCI | |
oaire.fundingStream | 3599-PPCDT | |
person.familyName | F. Duque | |
person.familyName | Bessa | |
person.familyName | Carvalho | |
person.familyName | Castro | |
person.givenName | Anouk | |
person.givenName | Vânia | |
person.givenName | M. Fátima | |
person.givenName | Paula | |
person.identifier | 2013444 | |
person.identifier.ciencia-id | 7819-FA0D-FC04 | |
person.identifier.ciencia-id | 6913-1464-155B | |
person.identifier.ciencia-id | 7C1F-6C72-354A | |
person.identifier.orcid | 0000-0002-8360-1404 | |
person.identifier.orcid | 0000-0002-2676-7184 | |
person.identifier.orcid | 0000-0002-7181-0540 | |
person.identifier.orcid | 0000-0001-8841-6606 | |
person.identifier.rid | N-5863-2014 | |
person.identifier.rid | K-8029-2014 | |
person.identifier.rid | M-8241-2013 | |
person.identifier.scopus-author-id | 36573459300 | |
person.identifier.scopus-author-id | 35214245200 | |
person.identifier.scopus-author-id | 14038677200 | |
person.identifier.scopus-author-id | 7102781782 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
rcaap.rights | restrictedAccess | por |
rcaap.type | article | por |
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