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Distribution and characterization of Listeria monocytogenes clinical isolates in Portugal, 1994–2007

2010Journal article Open access
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dc.contributor.authorAlmeida, G.
dc.contributor.authorMorvan, A.
dc.contributor.authorMagalhães, R.
dc.contributor.authorSantos, I.
dc.contributor.authorHogg, T.
dc.contributor.authorLeclercq, A.
dc.contributor.authorTeixeira, P.
dc.date.accessioned2011-08-01T16:12:52Z
dc.date.available2011-08-01T16:12:52Z
dc.date.issued2010
dc.description.abstractIn recent years, the number of cases of listeriosis has increased worldwide. Ninety-five isolates of Listeria monocytogenes recovered from Portuguese human cases of listeriosis have been characterized by biotyping (cadmium and arsenic sensitivity), polymerase chain reaction (PCR) grouping, and by pulsed-field gel electrophoresis (PFGE) applying the enzymes AscI and ApaI. Isolates were classified into one of three PCR groups; IVb (71.6%), IIb (17.9%), and IIa (10.5%). Four biotypes were differentiated: sensitive to arsenic/cadmium (48.4%), arsenic-sensitive and cadmium-resistant (25.3%), resistant to arsenic and sensitive to cadmium (18.9%), and resistant to both heavy metals (7.4%). Combined analyses of AscI and ApaI patterns yielded a total of 58 PFGE types with five sets (G, Jb, KKa, Me, and U) of Portuguese strains, each of which were indistinguishable by PFGE typing. In the present study, it was demonstrated that there are recurrent pulsotypes and that some were the same pulsotypes linked to outbreaks in France. In addition, there are some pulsotypes spread throughout the country, while others only appear in a restricted region. This study allowed the assembly of a first large pulsotype database of Portuguese clinical strains.por
dc.description.versioninfo:eu-repo/semantics/acceptedVersion
dc.identifier.citationALMEIDA, G... [et al.] - Distribution and characterization of Listeria monocytogenes clinical isolates in Portugal, 1994–2007. European Journal of Clinical Microbiology and Infectious Diseases. ISSN 0934-9723. Vol. 29, n.º 10 (2010), p. 1219-1227por
dc.identifier.doi10.1007/s10096-010-0988-x
dc.identifier.eissn1435-4373
dc.identifier.issn0934-9723
dc.identifier.urihttp://hdl.handle.net/10400.14/4900
dc.identifier.wosWOS:000281717900005
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringer Verlagpor
dc.relation96780
dc.titleDistribution and characterization of Listeria monocytogenes clinical isolates in Portugal, 1994–2007por
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-ALI%2F64662%2F2006/PT
oaire.citation.endPage1227
oaire.citation.issue10
oaire.citation.startPage1219
oaire.citation.titleEuropean Journal of Clinical Microbiology and Infectious Diseases
oaire.citation.volume29
oaire.fundingStream3599-PPCDT
person.familyNameAlmeida
person.familyNameMagalhães
person.familyNameHogg
person.familyNameLeclercq
person.familyNameTeixeira
person.givenNameGonçalo
person.givenNameRui
person.givenNameTim
person.givenNameAlexandre
person.givenNamePaula
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person.identifier.scopus-author-id7005603860
person.identifier.scopus-author-id55535974500
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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