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Molecular characterization of quinolone resistance mechanisms and extended-spectrum β-lactamase production in Escherichia coli isolated from dogs

2015Journal article Restricted access
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dc.contributor.authorMeireles, D.
dc.contributor.authorLeite-Martins, L.
dc.contributor.authorBessa, L. J.
dc.contributor.authorCunha, S.
dc.contributor.authorFernandes, R.
dc.contributor.authorMatos, A. de
dc.contributor.authorManaia, C. M.
dc.contributor.authorCosta, P. Martins da
dc.date.accessioned2016-04-21T16:33:36Z
dc.date.available2016-04-21T16:33:36Z
dc.date.issued2015
dc.description.abstractThe increasing prevalence of antimicrobial resistances is now a worldwide problem. Investigating the mechanisms by which pets harboring resistant strains may receive and/or transfer resistance determinants is essential to better understanding how owners and pets can interact safely. Here, we characterized the genetic determinants conferring resistance to β-lactams and quinolones in 38 multidrug-resistant Escherichia coli isolated from fecal samples of dogs, through PCR and sequencing. The most frequent geno-type included the β-lactamase groups TEM (n = 5), and both TEM + CTX-M-1 (n = 5). Within the CTX-Mgroup, we identified the genes CTX-M-32, CTX-M-1, CTX-M-15, CTX-M-55/79, CTX-M-14 and CTX-M-2/44. Thirty isolates resistant to ciprofloxacin presented two mutations in the gyrA gene and one or two mutations in the par C gene. A mutation in gyr A (reported here for the first time), due to a transversion andtransition (TCG → GTG) originating a substitution of a serine by a valine in position 83 was also detected.The plasmid-encoded quinolone resistance gene, qnrs1, was detected in three isolates. Dogs can be a reservoir of genetic determinants conferring antimicrobial resistance and thus may play an important role in the spread of antimicrobial resistance to humans and other co-habitant animals.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationMEIRELES, D… [et al.] - Molecular characterization of quinolone resistance mechanisms and extended-spectrum β-lactamase production in Escherichia coli isolated from dogs. Comparative Immunology, Microbiology and Infectious Diseases. ISSN 0147-9571. Vol. 41 (2015), p. 43-48pt_PT
dc.identifier.doi10.1016/j.cimid.2015.04.004
dc.identifier.eid84938999678
dc.identifier.eissn1878-1667
dc.identifier.issn0147-9571
dc.identifier.pmid25999092
dc.identifier.urihttp://hdl.handle.net/10400.14/19935
dc.identifier.wos000359297900006
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.relationDYNAMICS OF ANTIMICROBIAL RESISTANCE BETWEEN THE HOSPITAL AND THE ENVIRONMENT
dc.subjectDogspt_PT
dc.subjectEscherichia colipt_PT
dc.subjectAntimicrobial resistancept_PT
dc.subjectβ-lactamasept_PT
dc.subjectQuinolonesapt_PT
dc.titleMolecular characterization of quinolone resistance mechanisms and extended-spectrum β-lactamase production in Escherichia coli isolated from dogspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDYNAMICS OF ANTIMICROBIAL RESISTANCE BETWEEN THE HOSPITAL AND THE ENVIRONMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAC-AMB%2F113840%2F2009/PT
oaire.citation.endPage48
oaire.citation.startPage43
oaire.citation.titleComparative Immunology, Microbiology and Infectious Diseases
oaire.citation.volume41
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameCunha
person.familyNameFernandes
person.familyNameManaia
person.familyNameMartins da Costa
person.givenNameSara
person.givenNameRúben
person.givenNameCélia
person.givenNamePaulo
person.identifier509424
person.identifier.ciencia-idA610-969C-E6D7
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.ciencia-idC31F-553B-6365
person.identifier.ciencia-id3517-834C-6370
person.identifier.orcid0000-0003-1336-9993
person.identifier.orcid0000-0001-8933-3984
person.identifier.orcid0000-0002-3674-1789
person.identifier.orcid0000-0001-6115-8811
person.identifier.ridL-5753-2013
person.identifier.ridC-3080-2014
person.identifier.scopus-author-id14043440800
person.identifier.scopus-author-id6602465318
person.identifier.scopus-author-id56372529300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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