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In vitro ACE-inhibitory peptide KGYGGVSLPEW facilitates noradrenaline release from sympathetic nerve terminals: relationship with the lack of antihypertensive effect on spontaneous hypertensive rats

dc.contributor.authorMarques, Cláudia
dc.contributor.authorAmorim, Maria Manuela
dc.contributor.authorPereira, Joana Odila
dc.contributor.authorGuardão, Luísa
dc.contributor.authorMartins, Maria João
dc.contributor.authorPintado, Manuela Estevez
dc.contributor.authorMoura, Daniel
dc.contributor.authorCalhau, Conceição
dc.contributor.authorPinheiro, Hélder
dc.date.accessioned2016-01-07T15:57:50Z
dc.date.available2016-01-07T15:57:50Z
dc.date.issued2015
dc.description.abstractThis study aimed to validate the antihypertensive activity of the angiotensin-converting enzyme (ACE)-inhibitor whey protein hydrolysate (WPH) obtained through the action of proteolytic enzymes fromCynara Cardunculus. The antihypertensive activity of WPH fractions containing peptides with molecularweight below 3 kDa (Whey < 3 kDa) and 1 kDa (Whey < 1 kDa) along with the antihypertensive activity ofthree potent ACE-inhibitory peptide sequences (DKVGINYW, DAQSAPLRVY and KGYGGVSLPEW), previ-ously identified in WPH, were also investigated. In parallel, the influence of KGYGGVSLPEW (the mostpotent ACE-inhibitory peptide sequence) on AT1receptors (a common pharmacological target of anti-hypertensive therapies beyond ACE), was evaluated. The effect of WPH and fractions (300 mg/kg) andpeptide sequences (5 mg/kg) on systolic, diastolic and mean blood pressure was evaluated by telemetryon Spontaneously Hypertensive Rats (SHR), after single oral administration. Despite their ACE-inhibitoryeffect in vitro, neither WPH, Whey <3 kDa, Whey <1 kDa or peptide sequences exhibited antihyperten-sive activity. In addition, KGYGGVSLPEW was not only devoid of AT1receptor antagonism but, on thecontrary, had a similar effect to that of Ang II by facilitating the noradrenaline release from sympatheticnerve terminals. In vitro ACE blockade does not always correlate with antihypertensive activity and food-derived peptides cannot be classified as antihypertensive agents based exclusively on in vitro assays. Theabsence of an antihypertensive effect may also be a result of the interaction of these compounds withother components of the systems involved in the blood pressure control.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationMARQUES, Cláudia; AMORIM, Maria Manuela; PEREIRA, Joana Odila; GUARDÃO, Luísa; MARTINS, Maria João; PINTADO, M. E.; MOURA, Daniel; CALHAU, Conceição; PINHEIRO, Hélder - In vitro ACE-inhibitory peptide KGYGGVSLPEW facilitates noradrenaline release from sympathetic nerve terminals: Relationship with the lack of antihypertensive effect on spontaneous hypertensive rats. Peptides. ISSN 0196-9781. (2015) vol.71, p.72-76pt_PT
dc.identifier.doi10.1016/j.peptides.2015.06.005pt_PT
dc.identifier.eid84937239479
dc.identifier.eissn1873-5169
dc.identifier.issn0196-9781
dc.identifier.pmid26116781
dc.identifier.urihttp://hdl.handle.net/10400.14/18850
dc.identifier.wos000361837800010
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationQREN-ADI 11531pt_PT
dc.subjectAntihypertensivept_PT
dc.subjectAngiotensin-converting enzymept_PT
dc.subjectBioactive peptidespt_PT
dc.subjectHypertensionpt_PT
dc.subjectMechanisms of actionpt_PT
dc.subjectWheypt_PT
dc.titleIn vitro ACE-inhibitory peptide KGYGGVSLPEW facilitates noradrenaline release from sympathetic nerve terminals: relationship with the lack of antihypertensive effect on spontaneous hypertensive ratspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage76
oaire.citation.startPage72
oaire.citation.titlePeptides
oaire.citation.volume71
person.familyNameMarques
person.familyNameAmorim
person.familyNameOdila Pereira
person.familyNameMartins
person.familyNamePintado
person.familyNameMoura
person.familyNameCalhau
person.familyNamePinheiro
person.givenNameCláudia
person.givenNameMaria Manuela
person.givenNameJoana
person.givenNameMaria João
person.givenNameMaria Manuela
person.givenNameDaniel
person.givenNameConceição
person.givenNameHelder
person.identifier2343777
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person.identifier.ridL-4327-2014
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person.identifier.scopus-author-id55293251500
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rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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