Publication
In vitro ACE-inhibitory peptide KGYGGVSLPEW facilitates noradrenaline release from sympathetic nerve terminals: relationship with the lack of antihypertensive effect on spontaneous hypertensive rats
dc.contributor.author | Marques, Cláudia | |
dc.contributor.author | Amorim, Maria Manuela | |
dc.contributor.author | Pereira, Joana Odila | |
dc.contributor.author | Guardão, Luísa | |
dc.contributor.author | Martins, Maria João | |
dc.contributor.author | Pintado, Manuela Estevez | |
dc.contributor.author | Moura, Daniel | |
dc.contributor.author | Calhau, Conceição | |
dc.contributor.author | Pinheiro, Hélder | |
dc.date.accessioned | 2016-01-07T15:57:50Z | |
dc.date.available | 2016-01-07T15:57:50Z | |
dc.date.issued | 2015 | |
dc.description.abstract | This study aimed to validate the antihypertensive activity of the angiotensin-converting enzyme (ACE)-inhibitor whey protein hydrolysate (WPH) obtained through the action of proteolytic enzymes fromCynara Cardunculus. The antihypertensive activity of WPH fractions containing peptides with molecularweight below 3 kDa (Whey < 3 kDa) and 1 kDa (Whey < 1 kDa) along with the antihypertensive activity ofthree potent ACE-inhibitory peptide sequences (DKVGINYW, DAQSAPLRVY and KGYGGVSLPEW), previ-ously identified in WPH, were also investigated. In parallel, the influence of KGYGGVSLPEW (the mostpotent ACE-inhibitory peptide sequence) on AT1receptors (a common pharmacological target of anti-hypertensive therapies beyond ACE), was evaluated. The effect of WPH and fractions (300 mg/kg) andpeptide sequences (5 mg/kg) on systolic, diastolic and mean blood pressure was evaluated by telemetryon Spontaneously Hypertensive Rats (SHR), after single oral administration. Despite their ACE-inhibitoryeffect in vitro, neither WPH, Whey <3 kDa, Whey <1 kDa or peptide sequences exhibited antihyperten-sive activity. In addition, KGYGGVSLPEW was not only devoid of AT1receptor antagonism but, on thecontrary, had a similar effect to that of Ang II by facilitating the noradrenaline release from sympatheticnerve terminals. In vitro ACE blockade does not always correlate with antihypertensive activity and food-derived peptides cannot be classified as antihypertensive agents based exclusively on in vitro assays. Theabsence of an antihypertensive effect may also be a result of the interaction of these compounds withother components of the systems involved in the blood pressure control. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | |
dc.identifier.citation | MARQUES, Cláudia; AMORIM, Maria Manuela; PEREIRA, Joana Odila; GUARDÃO, Luísa; MARTINS, Maria João; PINTADO, M. E.; MOURA, Daniel; CALHAU, Conceição; PINHEIRO, Hélder - In vitro ACE-inhibitory peptide KGYGGVSLPEW facilitates noradrenaline release from sympathetic nerve terminals: Relationship with the lack of antihypertensive effect on spontaneous hypertensive rats. Peptides. ISSN 0196-9781. (2015) vol.71, p.72-76 | pt_PT |
dc.identifier.doi | 10.1016/j.peptides.2015.06.005 | pt_PT |
dc.identifier.eid | 84937239479 | |
dc.identifier.eissn | 1873-5169 | |
dc.identifier.issn | 0196-9781 | |
dc.identifier.pmid | 26116781 | |
dc.identifier.uri | http://hdl.handle.net/10400.14/18850 | |
dc.identifier.wos | 000361837800010 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | QREN-ADI 11531 | pt_PT |
dc.subject | Antihypertensive | pt_PT |
dc.subject | Angiotensin-converting enzyme | pt_PT |
dc.subject | Bioactive peptides | pt_PT |
dc.subject | Hypertension | pt_PT |
dc.subject | Mechanisms of action | pt_PT |
dc.subject | Whey | pt_PT |
dc.title | In vitro ACE-inhibitory peptide KGYGGVSLPEW facilitates noradrenaline release from sympathetic nerve terminals: relationship with the lack of antihypertensive effect on spontaneous hypertensive rats | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.citation.endPage | 76 | |
oaire.citation.startPage | 72 | |
oaire.citation.title | Peptides | |
oaire.citation.volume | 71 | |
person.familyName | Marques | |
person.familyName | Amorim | |
person.familyName | Odila Pereira | |
person.familyName | Martins | |
person.familyName | Pintado | |
person.familyName | Moura | |
person.familyName | Calhau | |
person.familyName | Pinheiro | |
person.givenName | Cláudia | |
person.givenName | Maria Manuela | |
person.givenName | Joana | |
person.givenName | Maria João | |
person.givenName | Maria Manuela | |
person.givenName | Daniel | |
person.givenName | Conceição | |
person.givenName | Helder | |
person.identifier | 2343777 | |
person.identifier | R-000-BVW | |
person.identifier | 456608 | |
person.identifier.ciencia-id | 791D-E9BA-D432 | |
person.identifier.ciencia-id | 9F15-0B2E-3DE8 | |
person.identifier.ciencia-id | 5413-5817-CE93 | |
person.identifier.ciencia-id | 2F13-AAE0-3405 | |
person.identifier.ciencia-id | 2110-0420-1717 | |
person.identifier.ciencia-id | FA13-B4AD-4BBB | |
person.identifier.orcid | 0000-0002-8365-7934 | |
person.identifier.orcid | 0000-0002-1485-0423 | |
person.identifier.orcid | 0000-0003-0300-5635 | |
person.identifier.orcid | 0000-0002-3560-3261 | |
person.identifier.orcid | 0000-0002-0760-3184 | |
person.identifier.orcid | 0000-0003-3848-3362 | |
person.identifier.orcid | 0000-0001-9567-3379 | |
person.identifier.orcid | 0000-0001-6076-2670 | |
person.identifier.rid | L-4327-2014 | |
person.identifier.rid | F-5696-2013 | |
person.identifier.scopus-author-id | 55293251500 | |
person.identifier.scopus-author-id | 57190164112 | |
person.identifier.scopus-author-id | 7402262295 | |
person.identifier.scopus-author-id | 7004483898 | |
person.identifier.scopus-author-id | 7005556838 | |
person.identifier.scopus-author-id | 6701578877 | |
person.identifier.scopus-author-id | 36931506900 | |
rcaap.rights | restrictedAccess | pt_PT |
rcaap.type | article | pt_PT |
relation.isAuthorOfPublication | 53f75876-3bef-4eb0-9fad-18d0f5a82fb6 | |
relation.isAuthorOfPublication | 70acb800-589a-40e5-9313-b004901b3752 | |
relation.isAuthorOfPublication | aaf32961-3b51-4d22-8932-5fbfabefcabd | |
relation.isAuthorOfPublication | 0924e116-1135-45c1-aaed-497386bdbe12 | |
relation.isAuthorOfPublication | ba387c7d-27c9-4016-895c-b35597e91ebc | |
relation.isAuthorOfPublication | b6aeeab9-aa3e-41e0-a776-d799d599d2d6 | |
relation.isAuthorOfPublication | 5198a1f6-6e2e-4951-ad26-2e93dae6596f | |
relation.isAuthorOfPublication | 1820a373-6184-498c-a859-17a6087fa366 | |
relation.isAuthorOfPublication.latestForDiscovery | 5198a1f6-6e2e-4951-ad26-2e93dae6596f |