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In vitro ACE-inhibitory peptide KGYGGVSLPEW facilitates noradrenaline release from sympathetic nerve terminals: relationship with the lack of antihypertensive effect on spontaneous hypertensive rats

2015Journal article Restricted access
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dc.contributor.authorMarques, Cláudia
dc.contributor.authorAmorim, Maria Manuela
dc.contributor.authorPereira, Joana Odila
dc.contributor.authorGuardão, Luísa
dc.contributor.authorMartins, Maria João
dc.contributor.authorPintado, Manuela Estevez
dc.contributor.authorMoura, Daniel
dc.contributor.authorCalhau, Conceição
dc.contributor.authorPinheiro, Hélder
dc.date.accessioned2016-01-07T15:57:50Z
dc.date.available2016-01-07T15:57:50Z
dc.date.issued2015
dc.description.abstractThis study aimed to validate the antihypertensive activity of the angiotensin-converting enzyme (ACE)-inhibitor whey protein hydrolysate (WPH) obtained through the action of proteolytic enzymes fromCynara Cardunculus. The antihypertensive activity of WPH fractions containing peptides with molecularweight below 3 kDa (Whey < 3 kDa) and 1 kDa (Whey < 1 kDa) along with the antihypertensive activity ofthree potent ACE-inhibitory peptide sequences (DKVGINYW, DAQSAPLRVY and KGYGGVSLPEW), previ-ously identified in WPH, were also investigated. In parallel, the influence of KGYGGVSLPEW (the mostpotent ACE-inhibitory peptide sequence) on AT1receptors (a common pharmacological target of anti-hypertensive therapies beyond ACE), was evaluated. The effect of WPH and fractions (300 mg/kg) andpeptide sequences (5 mg/kg) on systolic, diastolic and mean blood pressure was evaluated by telemetryon Spontaneously Hypertensive Rats (SHR), after single oral administration. Despite their ACE-inhibitoryeffect in vitro, neither WPH, Whey <3 kDa, Whey <1 kDa or peptide sequences exhibited antihyperten-sive activity. In addition, KGYGGVSLPEW was not only devoid of AT1receptor antagonism but, on thecontrary, had a similar effect to that of Ang II by facilitating the noradrenaline release from sympatheticnerve terminals. In vitro ACE blockade does not always correlate with antihypertensive activity and food-derived peptides cannot be classified as antihypertensive agents based exclusively on in vitro assays. Theabsence of an antihypertensive effect may also be a result of the interaction of these compounds withother components of the systems involved in the blood pressure control.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationMARQUES, Cláudia; AMORIM, Maria Manuela; PEREIRA, Joana Odila; GUARDÃO, Luísa; MARTINS, Maria João; PINTADO, M. E.; MOURA, Daniel; CALHAU, Conceição; PINHEIRO, Hélder - In vitro ACE-inhibitory peptide KGYGGVSLPEW facilitates noradrenaline release from sympathetic nerve terminals: Relationship with the lack of antihypertensive effect on spontaneous hypertensive rats. Peptides. ISSN 0196-9781. (2015) vol.71, p.72-76pt_PT
dc.identifier.doi10.1016/j.peptides.2015.06.005pt_PT
dc.identifier.eid84937239479
dc.identifier.eissn1873-5169
dc.identifier.issn0196-9781
dc.identifier.pmid26116781
dc.identifier.urihttp://hdl.handle.net/10400.14/18850
dc.identifier.wos000361837800010
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationQREN-ADI 11531pt_PT
dc.subjectAntihypertensivept_PT
dc.subjectAngiotensin-converting enzymept_PT
dc.subjectBioactive peptidespt_PT
dc.subjectHypertensionpt_PT
dc.subjectMechanisms of actionpt_PT
dc.subjectWheypt_PT
dc.titleIn vitro ACE-inhibitory peptide KGYGGVSLPEW facilitates noradrenaline release from sympathetic nerve terminals: relationship with the lack of antihypertensive effect on spontaneous hypertensive ratspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage76
oaire.citation.startPage72
oaire.citation.titlePeptides
oaire.citation.volume71
person.familyNameMarques
person.familyNameAmorim
person.familyNameOdila Pereira
person.familyNameMartins
person.familyNamePintado
person.familyNameMoura
person.familyNameCalhau
person.familyNamePinheiro
person.givenNameCláudia
person.givenNameMaria Manuela
person.givenNameJoana
person.givenNameMaria João
person.givenNameMaria Manuela
person.givenNameDaniel
person.givenNameConceição
person.givenNameHelder
person.identifier2343777
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person.identifier.orcid0000-0002-8365-7934
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person.identifier.orcid0000-0003-0300-5635
person.identifier.orcid0000-0002-3560-3261
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0003-3848-3362
person.identifier.orcid0000-0001-9567-3379
person.identifier.orcid0000-0001-6076-2670
person.identifier.ridL-4327-2014
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person.identifier.scopus-author-id55293251500
person.identifier.scopus-author-id57190164112
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rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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