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Abstract(s)
De todos os catiões presentes no corpo, o magnésio é o segundo mais importante catião e o quarto mais prevalente. Doenças que envolvem o magnésio são classificadas em dois grupos: hipomagnesemia (défice de magnésio) e hipermagnesemia. Desta forma, a determinação de magnésio despertou grande interesse, porque auxilia no contexto clínico e em pesquisas epidemiológicas. Portanto, o objetivo deste trabalho foi desenvolver um dispositivo microfluídico analítico em papel (μPAD) para quantificar magnésio em amostras salivares. Neste caso em concreto, o μPAD baseia-se numa reação colorimétrica entre o magnésio e o eriocrómio de cianina, formando uma cor laranja/avermelhada intensa. Após a reação, é necessário utilizar um scanner de mesa para obter uma imagem de alta resolução da zona de deteção do μPAD para determinar a intensidade de cor laranja/avermelhada de cada unidade de teste, medida através do software Image J. Sob condições ótimas, o método para o μPAD proposto foi caracterizado por um intervalo de calibração para a concentração de magnésio entre 0.082 – 0.247 mmol/L. Os limites de deteção e quantificação foram 0.062 mM e 0.081 mM, respetivamente. O gasto dos reagentes, eriocrómio cianina, NH4Cl e NH4OH foram 0.043 mg, 1.62 mg e 13.0 mg por curva de calibração. O gasto da solução padrão/amostra foi 120 μl por cada determinação.
Of all the cations in the body, magnesium is the second most important intracellular cation and the fourth most prevalent. Disorders involving magnesium are categorized into two groups: hypomagnesemia (magnesium deficiency) and hypermagnesemia. In this way, the determination of magnesium has aroused great interest, because it helps in the clinical context and epidemiological research. Therefore, the objective of this work was to develop a microfluidic paper-based analytical device (μPAD) for the quantification of magnesium in saliva samples. In this case, the μPAD is based on the colorimetric reaction between magnesium and eriochrome cyanine to form an intense orange/reddish dye. After the reaction, it is necessary to use a flatbed scanner to obtain a high-resolution image of the detection zone for determination the intensity of the orange/reddish colour within each detection zone measured with Image J software. Under the optimum conditions, the proposed μPAD method was characterized by a linear calibration range for magnesium concentration 0.082 – 0.247 mmol/L. The detection and quantification limits were 0.062 mM and 0.081 mM, respectively. The reagents, eriochrome cyanine, NH4Cl and NH4OH consumption were 0.043 mg, 1.62 mg and 13.0 mg per calibration curve and the sample consumption was 120 μl per eachdetermination.
Of all the cations in the body, magnesium is the second most important intracellular cation and the fourth most prevalent. Disorders involving magnesium are categorized into two groups: hypomagnesemia (magnesium deficiency) and hypermagnesemia. In this way, the determination of magnesium has aroused great interest, because it helps in the clinical context and epidemiological research. Therefore, the objective of this work was to develop a microfluidic paper-based analytical device (μPAD) for the quantification of magnesium in saliva samples. In this case, the μPAD is based on the colorimetric reaction between magnesium and eriochrome cyanine to form an intense orange/reddish dye. After the reaction, it is necessary to use a flatbed scanner to obtain a high-resolution image of the detection zone for determination the intensity of the orange/reddish colour within each detection zone measured with Image J software. Under the optimum conditions, the proposed μPAD method was characterized by a linear calibration range for magnesium concentration 0.082 – 0.247 mmol/L. The detection and quantification limits were 0.062 mM and 0.081 mM, respectively. The reagents, eriochrome cyanine, NH4Cl and NH4OH consumption were 0.043 mg, 1.62 mg and 13.0 mg per calibration curve and the sample consumption was 120 μl per eachdetermination.
Description
Keywords
Saliva Magnésio Dispositivo microfluídico analítico baseado em papel (μPAD) Magnesium Microfluidic paper-based analytical device (μPAD)