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Optical fiber bioanalyzer based on enzymatic coating matrix for catecholamines and their metabolites assessment in patients with down syndrome

2012Journal article Restricted access
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dc.contributor.authorSilva, Lurdes I. B.
dc.contributor.authorGomes, A. M.
dc.contributor.authorPintado, M. M.
dc.contributor.authorPinheiro, H.
dc.contributor.authorMoura, D.
dc.contributor.authorFreitas, Ana C.
dc.contributor.authorRocha-Santos, Teresa A. P.
dc.contributor.authorPereira, M. E.
dc.contributor.authorDuarte, A. C.
dc.date.accessioned2013-01-28T10:41:29Z
dc.date.available2013-01-28T10:41:29Z
dc.date.issued2012
dc.description.abstractThe urinary levels of catecholamines [adrenaline (AD), noradrenaline (NA), and dopamine (DA)] and their metabolites [L-3,4-dihydroxyphenylalanine (L-DOPA), and 3,4-dihydroxyphenylacetic acid (DOPAC)], as indicators of physiological stress, were assessed in 40 patients with Down syndrome (DS). The analysis was performed by an optical fiber (OF) bioanalyzer; which was constituted by two main components: a miniaturized chromatographic system and a detection system based on an OF coated with an enzymatic matrix. In this study some working conditions such as, number of fibers in the miniaturized chromatographic column, number of dips for sensitive coating deposition, temperature and time of cure of the sensitive matrix, were optimized in order to achieve higher analytical performance. After tested for calibration the bioanalyzer was applied to urine samples analysis of catecholamines and their metabolites, comparing the results with those obtained by both, a classical analytical method, namely high performance liquid chromatography coupled to an electrochemical detector (HPLC-ED), and an OF biosensor based on a sensitive cladding of laccase (LacOF biosensor). The results of catecholamines in patients with DS revealed that 15% and 22.5% of the analyzed subjects showed DA and AD concentrations, respectively, above the pathological levels. In the determinations of NA, the 40 samples showed concentration values below the normal levels, while in the determination of catecholamines metabolites 5% of the urine samples showed values above 118 and 1681 for L-DOPA and DOPAC, respectively. Index Terms—Catecholamines analysis, down syndrome, HPLC-ED, OF bioanalyzer.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationSILVA, Lurdes I. B... [et al.] - Optical Fiber Bioanalyzer Based on Enzymatic Coating Matrix for Catecholamines and Their Metabolites Assessment in Patients With Down Syndrome. IEEE Sensors Journal. ISSN 1530-437X. Vol. 12, n.º 1 (2012), p. 76-84por
dc.identifier.doi10.1109/JSEN.2011.2127471
dc.identifier.eissn1558-1748
dc.identifier.issn1530-437X
dc.identifier.urihttp://hdl.handle.net/10400.14/9958
dc.identifier.wosWOS:000297631700013
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherIEEEpor
dc.titleOptical fiber bioanalyzer based on enzymatic coating matrix for catecholamines and their metabolites assessment in patients with down syndromepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage84
oaire.citation.issue1
oaire.citation.startPage76
oaire.citation.titleIEEE Sensors Journal
oaire.citation.volume12
person.familyNameGomes
person.familyNamePintado
person.familyNamePinheiro
person.familyNameMoura
person.familyNameCardoso Freitas Lopes de Freitas
person.familyNameRocha-Santos
person.familyNamePereira
person.familyNameDuarte
person.givenNameAna Maria
person.givenNameMaria Manuela
person.givenNameHelder
person.givenNameDaniel
person.givenNameAna Cristina
person.givenNameTeresa
person.givenNameEduarda
person.givenNameArmando
person.identifier162628
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person.identifier.orcid0000-0002-1430-9370
person.identifier.orcid0000-0003-3660-4116
person.identifier.orcid0000-0002-6046-5243
person.identifier.orcid0000-0002-4868-4099
person.identifier.ridB-9944-2013
person.identifier.ridF-5696-2013
person.identifier.ridA-2355-2009
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person.identifier.scopus-author-id57190668768
person.identifier.scopus-author-id7004483898
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rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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