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DNA agarose gel electrophoresis for antioxidant analysis: Development of a quantitative approach for phenolic extracts

2017Journal article Restricted access
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dc.contributor.authorSilva, Sara
dc.contributor.authorCosta, Eduardo M.
dc.contributor.authorVicente, Sandra
dc.contributor.authorVeiga, Mariana
dc.contributor.authorCalhau, Conceição
dc.contributor.authorMorais, Rui M.
dc.contributor.authorPintado, Manuela E.
dc.date.accessioned2017-12-19T18:20:59Z
dc.date.available2017-12-19T18:20:59Z
dc.date.issued2017
dc.description.abstractMost of the fast in vitro assays proposed to determine the antioxidant capacity of a compound/extract lack either biological context or employ complex protocols. Therefore, the present work proposes the improvement of an agarose gel DNA electrophoresis in order to allow for a quantitative estimation of the antioxidant capacity of pure phenolic compounds as well as of a phenolic rich extract, while also considering their possible pro-oxidant effects. The result obtained demonstrated that the proposed method allowed for the evaluation of the protection of DNA oxidation [in the presence of hydrogen peroxide (H2O2) and an H2O2/iron (III) chloride (FeCl3) systems] as well as for the observation of pro-oxidant activities, with the measurements registering interclass correlation coefficients above 0.9. Moreover, this method allowed for the characterization of the antioxidant capacity of a blueberry extract while demonstrating that it had no perceived pro-oxidant effect.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, S., Costa, E. M., Vicente, S., Veiga, M., Calhau, C., Morais, R. M. S. C., Pintado, M. E. (2017). DNA agarose gel electrophoresis for antioxidant analysis: Development of a quantitative approach for phenolic extracts. Food Chemistry, 233, 45-51pt_PT
dc.identifier.doi10.1016/j.foodchem.2017.04.075pt_PT
dc.identifier.eid85018498781
dc.identifier.eissn1873-7072
dc.identifier.issn0308-8146
dc.identifier.pmid28530597
dc.identifier.urihttp://hdl.handle.net/10400.14/23768
dc.identifier.wos000402343100006
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationTextile staining: Toward a greener and functional solution
dc.subjectAntioxidant assayspt_PT
dc.subjectPro-oxidant assayspt_PT
dc.subjectPhenolic compoundspt_PT
dc.subjectBlueberry extractpt_PT
dc.subjectAscorbic acidpt_PT
dc.subjectGallic acidpt_PT
dc.subjectDNA
dc.titleDNA agarose gel electrophoresis for antioxidant analysis: Development of a quantitative approach for phenolic extractspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTextile staining: Toward a greener and functional solution
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F90867%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBDE%2F103957%2F2014/PT
oaire.citation.endPage51pt_PT
oaire.citation.startPage45pt_PT
oaire.citation.titleFood Chemistrypt_PT
oaire.citation.volume233pt_PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameSilva
person.familyNameCosta
person.familyNameCalhau
person.familyNameMorais
person.familyNamePintado
person.givenNameSara
person.givenNameEduardo
person.givenNameConceição
person.givenNameRui M. S. C.
person.givenNameMaria Manuela
person.identifier1888852
person.identifier143926
person.identifier456608
person.identifier.ciencia-id8E18-0AFF-BF6D
person.identifier.ciencia-idB218-870B-98B5
person.identifier.ciencia-id2110-0420-1717
person.identifier.ciencia-id541B-04AE-0686
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0001-8268-2137
person.identifier.orcid0000-0003-3121-4514
person.identifier.orcid0000-0001-9567-3379
person.identifier.orcid0000-0001-5138-6941
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridM-5065-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id55158300800
person.identifier.scopus-author-id55249865700
person.identifier.scopus-author-id6701578877
person.identifier.scopus-author-id7005146997
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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