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Incorporation of Bifidobacterium animalis subspecies lactis BB-12® and Akkermansia muciniphila in chocolate matrices

dc.contributor.authorVedor, Rita
dc.contributor.authorMachado, Daniela
dc.contributor.authorBarbosa, Joana Cristina
dc.contributor.authorAlmeida, Diana
dc.contributor.authorGomes, Ana Maria
dc.date.accessioned2023-10-19T16:42:00Z
dc.date.available2023-10-19T16:42:00Z
dc.date.issued2023-09-15
dc.description.abstractChocolate is a food product highly popular worldwide, and it has been proposed as a carrier for probiotic delivery. This work evaluated the viability of probiotic strains, Bifidobacterium animalis subsp. lactis BB-12® and Akkermansia muciniphila DSM 22959, in chocolate matrices with different cocoa percentages (33.6; 54.5 and 70.5%) throughout 28-days of aerobic storage. The pH, total phenolic content and antioxidant, antidiabetic, and antihypertensive activities were also determined at timepoints of 0 and 28-days. During storage, all chocolates showed high pH stability (variations lower than 1 unit) and it was observed a growing trend in total phenolic content and in bioactivities with the increase of cocoa content in the matrix. Regarding probiotic viability, a higher level for B. animalis subsp. lactis BB-12® was achieved in chocolate containing 70.5% cocoa (estimated in 108 CFU/g after storage). In opposite, A muciniphila DSM 22959 exhibited greater viability in chocolate with 33.6% and 54.5% cocoa (around 106 CFU/g after storage, meeting the minimum required amounts for probiotic products). Conjugating the data from physicochemical properties, bioactivities and probiotic viability, dark chocolate matrices with 54.5% and 70.5% cocoa, may be considered a promising food vector for A. muciniphila DSM 22959 and B. animalis subsp. lactis BB-12®, respectively.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.lwt.2023.115361pt_PT
dc.identifier.eid85173170464
dc.identifier.issn0023-6438
dc.identifier.urihttp://hdl.handle.net/10400.14/42871
dc.identifier.wos001091386900001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBioactivitiespt_PT
dc.subjectCocoa contentpt_PT
dc.subjectProbiotic viabilitypt_PT
dc.subjectTotal phenolic contentpt_PT
dc.titleIncorporation of Bifidobacterium animalis subspecies lactis BB-12® and Akkermansia muciniphila in chocolate matricespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleLWTpt_PT
oaire.citation.volume187pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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