Publication
Incorporation of Bifidobacterium animalis subspecies lactis BB-12® and Akkermansia muciniphila in chocolate matrices
dc.contributor.author | Vedor, Rita | |
dc.contributor.author | Machado, Daniela | |
dc.contributor.author | Barbosa, Joana Cristina | |
dc.contributor.author | Almeida, Diana | |
dc.contributor.author | Gomes, Ana Maria | |
dc.date.accessioned | 2023-10-19T16:42:00Z | |
dc.date.available | 2023-10-19T16:42:00Z | |
dc.date.issued | 2023-09-15 | |
dc.description.abstract | Chocolate is a food product highly popular worldwide, and it has been proposed as a carrier for probiotic delivery. This work evaluated the viability of probiotic strains, Bifidobacterium animalis subsp. lactis BB-12® and Akkermansia muciniphila DSM 22959, in chocolate matrices with different cocoa percentages (33.6; 54.5 and 70.5%) throughout 28-days of aerobic storage. The pH, total phenolic content and antioxidant, antidiabetic, and antihypertensive activities were also determined at timepoints of 0 and 28-days. During storage, all chocolates showed high pH stability (variations lower than 1 unit) and it was observed a growing trend in total phenolic content and in bioactivities with the increase of cocoa content in the matrix. Regarding probiotic viability, a higher level for B. animalis subsp. lactis BB-12® was achieved in chocolate containing 70.5% cocoa (estimated in 108 CFU/g after storage). In opposite, A muciniphila DSM 22959 exhibited greater viability in chocolate with 33.6% and 54.5% cocoa (around 106 CFU/g after storage, meeting the minimum required amounts for probiotic products). Conjugating the data from physicochemical properties, bioactivities and probiotic viability, dark chocolate matrices with 54.5% and 70.5% cocoa, may be considered a promising food vector for A. muciniphila DSM 22959 and B. animalis subsp. lactis BB-12®, respectively. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
dc.identifier.doi | 10.1016/j.lwt.2023.115361 | pt_PT |
dc.identifier.eid | 85173170464 | |
dc.identifier.issn | 0023-6438 | |
dc.identifier.uri | http://hdl.handle.net/10400.14/42871 | |
dc.identifier.wos | 001091386900001 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
dc.subject | Bioactivities | pt_PT |
dc.subject | Cocoa content | pt_PT |
dc.subject | Probiotic viability | pt_PT |
dc.subject | Total phenolic content | pt_PT |
dc.title | Incorporation of Bifidobacterium animalis subspecies lactis BB-12® and Akkermansia muciniphila in chocolate matrices | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.citation.title | LWT | pt_PT |
oaire.citation.volume | 187 | pt_PT |
rcaap.rights | openAccess | pt_PT |
rcaap.type | article | pt_PT |