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Virulence factors among enterococci isolated from traditional fermented meat products produced in the North of Portugal

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dc.contributor.authorBarbosa, J.
dc.contributor.authorGibbs, P.A.
dc.contributor.authorTeixeira, P.
dc.date.accessioned2011-08-01T16:30:44Z
dc.date.available2011-08-01T16:30:44Z
dc.date.issued2010
dc.description.abstractThe aim of this work was to characterize Enterococcus spp. isolated from Alheira, Chouriça de Vinhais and Salpicão de Vinhais, fermented meat products produced in the North of Portugal, concerning their potential pathogenicity. One hundred and eighty two isolates (76 identified as Enterococcus faecalis, 44 as Enterococcus faecium, 1 as Enterococcus casseliflavus and 61 as Enterococcus spp.) were studied. Twenty six percent of isolates were gelatinase producers. None of the isolates produced lipase nor DNase activities. Hemolytic activity using sheep and human blood from two types (A and O) was assessed. One isolate was b-hemolytic in human blood. Results obtained in sheep blood were quite different from those obtained in human blood. Biofilm production in batch and in fed-batch mode was evaluated. In batch mode, only 28.0% and 3.9% of isolates were classified as moderate and strong biofilm producers, respectively, and in fed-batch mode, 35.7% and 63.2% of isolates were classified as moderate and strong biofilm producers, respectively. The presence of 13 virulence genes (efaAfs, efaAfm, esp, agg, cylM, cylB, cylA, cylLL cylLs and gelE) were investigated by PCR. The majority of enterococcal isolates showed the presence of one or more virulence factors, the most frequent genotype being efaAfs+ gelE+ agg+ (41.5%). E. faecalis isolates harbored multiple virulence traits, while E. faecium isolates were generally free of virulence determinants. Phenotypic and genotypic evidence of potential virulence factors were identified in Enterococcus spp. isolates, which is a reason of concern.por
dc.identifier.citationBARBOSA, J. ; GIBBS, P. A. ; TEIXEIRA, P. - Virulence factors among enterococci isolated from traditional fermented meat products produced in the North of Portugal. Food Control. ISSN 0956-7135. Vol. 21, n.º 5 (2010), p. 651-656por
dc.identifier.doi10.1016/j.foodcont.2009.10.002
dc.identifier.eissn1873-7129
dc.identifier.issn0956-7135
dc.identifier.urihttp://hdl.handle.net/10400.14/4913
dc.identifier.wosWOS:000274843400011
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationTradition, Quality & Safety- traditional fermented meat products.
dc.subjectFermented meat productspor
dc.subjectEnterococcipor
dc.subjectVirulence factorspor
dc.titleVirulence factors among enterococci isolated from traditional fermented meat products produced in the North of Portugalpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTradition, Quality & Safety- traditional fermented meat products.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FAGG%2F39587%2F2001/PT
oaire.citation.endPage656
oaire.citation.issue5
oaire.citation.startPage651
oaire.citation.titleFood Control
oaire.citation.volume21
oaire.fundingStreamPOCI
person.familyNameBarbosa
person.familyNameGibbs
person.familyNameTeixeira
person.givenNameJoana
person.givenNamePaul
person.givenNamePaula
person.identifier.ciencia-id9516-589F-8B0D
person.identifier.orcid0000-0001-5879-9458
person.identifier.orcid0000-0002-6580-4363
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id13608752600
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationce89919c-98a7-473c-995a-1d89e70644b6
relation.isAuthorOfPublication218d85c8-40f4-42c0-9a47-4e8ad9f72a96
relation.isAuthorOfPublicationb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isAuthorOfPublication.latestForDiscoveryb918d4a2-b9f9-4c56-8318-111625e4d58b
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relation.isProjectOfPublication.latestForDiscoverye5ddd817-2f7b-463f-b437-c0989abbd8b0

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