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Survival of clinical and food isolates of listeria monocytogenes through simulated gastrointestinal tract conditions

2010Journal article Restricted access
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dc.contributor.authorRamalheira, Rosário
dc.contributor.authorAlmeida, Marta
dc.contributor.authorAzeredo, Joana
dc.contributor.authorBrandão, Teresa R.S.
dc.contributor.authorAlmeida, Gonçalo
dc.contributor.authorSilva, Joana
dc.contributor.authorTeixeira, Paula
dc.date.accessioned2010-10-06T11:24:30Z
dc.date.available2010-10-06T11:24:30Z
dc.date.issued2010
dc.description.abstractTwenty-seven strains of Listeria monocytogenes previously isolated from food (n¼16) and human patients of listeriosis (n¼11) were characterized and compared based on their ability to survive through the simulated gastrointestinal tract conditions. Cells were exposed (60 or 120 min) to low pH in the presence of pepsin, to simulate the digestion in the stomach, and subsequently to bile salts to simulate the digestion in the small intestine (60 or 120 min). Their survival was shown to be origin- (food and clinical) and strain dependent ( p<0.001) and also significantly dependent on the imposed simulated gastric conditions (long vs. quick exposure) ( p<0.001). In comparison to the food isolates, the clinical strains were in general more resistant and survived better to the two challenges imposed. Some of the tested strains, after the exposure to low pH in the presence of pepsin, became injured and subsequently more susceptible to the bile salts challenge. It was demonstrated that one of the most important natural barriers against foodborne pathogens might not be effective since it was shown that L. monocytogenes isolates that survived through the pH challenge were also able to survive the subsequent challenge to bile salts.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citation"Foodborne Pathogens and Disease". ISSN 1535-3141. 7: 2 (2010) 121-128por
dc.identifier.doi10.1089/fpd.2009.0319
dc.identifier.eissn1556-7125
dc.identifier.issn1535-3141
dc.identifier.urihttp://hdl.handle.net/10400.14/2663
dc.identifier.wosWOS:000273862200002
dc.language.isoengpor
dc.publisherMary Ann Liebertpor
dc.relation2006-016264-Truefood
dc.titleSurvival of clinical and food isolates of listeria monocytogenes through simulated gastrointestinal tract conditionspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-ALI%2F64662%2F2006/PT
oaire.citation.endPage128
oaire.citation.issue2
oaire.citation.startPage121
oaire.citation.titleFoodborne Pathogens and Disease
oaire.citation.volume7
oaire.fundingStream3599-PPCDT
person.familyNameAzeredo
person.familyNameBrandão
person.familyNameAlmeida
person.familyNameSilva
person.familyNameTeixeira
person.givenNameJoana
person.givenNameTeresa
person.givenNameGonçalo
person.givenNameJoana
person.givenNamePaula
person.identifierJ-9418-2012
person.identifier267767
person.identifier.ciencia-id7B19-E23B-BB31
person.identifier.ciencia-id341E-5F76-548C
person.identifier.ciencia-idB415-743D-D9F9
person.identifier.orcid0000-0002-5180-7133
person.identifier.orcid0000-0002-8857-6471
person.identifier.orcid0000-0003-1513-0951
person.identifier.orcid0000-0003-3977-7418
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridJ-9418-2012
person.identifier.ridK-4389-2014
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id6701433514
person.identifier.scopus-author-id6602174128
person.identifier.scopus-author-id7003759404
person.identifier.scopus-author-id7403023130
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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