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Enantiomeric separation of tramadol and Its metabolites: method validation and application to environmental samples

2017Journal article Open access
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dc.contributor.authorSilva, Cátia
dc.contributor.authorRibeiro, Cláudia
dc.contributor.authorMaia, Alexandra S.
dc.contributor.authorGonçalves, Virgínia
dc.contributor.authorTiritan, Maria Elizabeth
dc.contributor.authorAfonso, Carlos
dc.date.accessioned2017-12-19T18:14:05Z
dc.date.available2017-12-19T18:14:05Z
dc.date.issued2017
dc.description.abstractThe accurate assessment of racemic pharmaceuticals requires enantioselective analytical methods. This study presents the development and validation of an enantioselective liquid chromatography with a fluorescence detection method for the concomitant quantification of the enantiomers of tramadol and their metabolites, N-desmethyltramadol and O-desmethyltramadol, in wastewater samples. Optimized conditions were achieved using a Lux Cellulose-4 column 150 × 4.6 mm, 3 μm isocratic elution, and 0.1% diethylamine in hexane and ethanol (96:4, v/v) at 0.7 mL min-1. The samples were extracted using 150 mg Oasis® mixed-mode cation exchange (MCX) cartridges. The method was validated using a synthetic effluent of a laboratory-scale aerobic granular sludge sequencing batch reactor. The method demonstrated to be selective, accurate, and linear (r2 > 0.99) over the range of 56 ng L-1 to 392 ng L-1. The detection and the quantification limits of each enantiomer were 8 ng L-1 and 28 ng L-1 for tramadol and N-desmethyltramadol, and 20 ng L-1 and 56 ng L-1 for O-desmethyltramadol. The feasibility of the method was demonstrated in a screening study in influent and effluent samples from a wastewater treatment plant. The results demonstrated the occurrence of tramadol enantiomers up to 325.1 ng L-1 and 357.9 ng L-1, in the effluent and influent samples, respectively. Both metabolites were detected in influents and effluents.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, C., Ribeiro, C., Maia, A. S., Goncalves, V., Tiritan, M. E., & Afonso, C. (2017). Enantiomeric separation of tramadol and its metabolites: Method validation and application to environmental samples. Symmetry-Basel, 9(9), 170pt_PT
dc.identifier.doi10.3390/sym9090170pt_PT
dc.identifier.eid85029418808
dc.identifier.issn2073-8994
dc.identifier.urihttp://hdl.handle.net/10400.14/23766
dc.identifier.wos000411526000003
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectChiral pharmaceuticalspt_PT
dc.subjectTramadolpt_PT
dc.subjectN-desmethyltramadolpt_PT
dc.subjectO-desmethyltramadolpt_PT
dc.subjectWastewaterpt_PT
dc.subjectLux Cellulose-4 columnpt_PT
dc.titleEnantiomeric separation of tramadol and Its metabolites: method validation and application to environmental samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04423%2F2013/PT
oaire.citation.issue9pt_PT
oaire.citation.titleSymmetrypt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream5876
person.familyNameRibeiro
person.familyNameMaia
person.familyNameFerreira Gonçalves
person.familyNameTiritan
person.familyNameAfonso
person.givenNameClaudia
person.givenNameAlexandra S.
person.givenNameVirgínia Maria
person.givenNameMaria Elizabeth
person.givenNameCarlos
person.identifier1310409
person.identifier.ciencia-id9D17-0C17-2703
person.identifier.ciencia-id6A15-F0A3-AEF2
person.identifier.ciencia-idDB12-D51F-C2D7
person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.ciencia-id6A10-3565-6158
person.identifier.orcid0000-0001-7245-4552
person.identifier.orcid0000-0002-4167-383X
person.identifier.orcid0000-0002-9151-516X
person.identifier.orcid0000-0003-3320-730X
person.identifier.orcid0000-0002-5731-5789
person.identifier.ridK-9492-2014
person.identifier.ridI-5989-2015
person.identifier.scopus-author-id56835095200
person.identifier.scopus-author-id55332349300
person.identifier.scopus-author-id23097687200
person.identifier.scopus-author-id6602890981
person.identifier.scopus-author-id36145043600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication85c78d96-4a73-4aac-9f9a-81fdb146263c
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