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Peptide-rich yeast fractions from brewer’s spent yeast: a scalable fractionation approach and their functional application in bakery products

dc.contributor.authorBrassesco, María Emilia
dc.contributor.authorPaupério, Ana
dc.contributor.authorPereira, Carlos D.
dc.contributor.authorFerreira, João Paulo
dc.contributor.authorPintado, Manuela
dc.date.accessioned2025-04-11T11:23:46Z
dc.date.available2025-04-11T11:23:46Z
dc.date.issued2025-03-25
dc.description.abstractBrewer’s spent yeast (BSY), a significant brewing by-product, poses environmental challenges and opportunities for valorization as a sustainable protein source. This study focuses on transforming BSY into high-value functional ingredients for food applications. A green, sustainable, and scalable process was developed to extract bioactive compounds from BSY at both laboratory and pilot scales, yielding peptide-rich fractions with robust antioxidant properties. These extracts were incorporated into prototype formulations, including protein-enriched crackers, demonstrating their potential as natural, nutritious ingredients. Physicochemical, compositional, and functional characterizations validated their application viability. The antioxidant potential of BSY fractions was confirmed through total phenolic compounds and ABTS and oxygen radical absorbance capacity assays, where the retentate from the 10 kDa ultrafiltration fraction on the pilot scale exhibited superior bioactivity, supporting its selection as the most suitable fraction for food formulations. Additionally, the transition from laboratory to pilot scale revealed slight variations in protein retention and bioactive compound recovery, emphasizing the need for process optimization. These findings highlight BSY’s potential to support circular economy practices by reducing waste while enhancing the nutritional and functional value of food products.eng
dc.identifier.doi10.3390/foods14071144
dc.identifier.eid105002387096
dc.identifier.issn2304-8158
dc.identifier.pmid40238259
dc.identifier.urihttp://hdl.handle.net/10400.14/52967
dc.identifier.wos001463816900001
dc.language.isoeng
dc.peerreviewedyes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectUltrafiltration process
dc.subjectProcess scale-up
dc.subjectFunctional ingredients
dc.subjectBy-product valorization
dc.subjectBioactive compounds
dc.titlePeptide-rich yeast fractions from brewer’s spent yeast: a scalable fractionation approach and their functional application in bakery productseng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.issue7
oaire.citation.titleFoods
oaire.citation.volume14
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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