Applied Molecular Biosciences Unit

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Publications

Lactobacillus mulieris sp. nov., a new species of Lactobacillus delbrueckii group

Publication . Rocha, Joana; Botelho, João; Ksiezarek, Magdalena; Ugarcina Perovic, Svetlana; Machado, Miguel; Carrico, João André; Pimentel, Lígia L.; Salsinha, Sofia; Rodríguez-Alcalá, Luis M.; Pintado, Manuela; Ribeiro, Teresa G.; Peixe, Luísa

One Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, and coccobacilli-shaped strain, designated c10Ua161MT, was isolated from a urine sample from a reproductive-age healthy woman. Comparative 16S rRNA gene sequence analysis indicated that strain c10Ua161MT belonged to the genus Lactobacillus . Phylogenetic analysis based on pheS and rpoA gene sequences strongly supported a clade encompassing strains c10Ua161MT and eight other strains from public databases, distinct from currently recognized species of the genus Lactobacillus. In silico Average Nucleotide Identity (ANI) and Genome-to-Genome Distance Calculator (GGDC), showed 87.9 and 34.3 % identity to the closest relative Lactobacillus jensenii , respectively. The major fatty acids of strain c10Ua161MT were C18 : 1ω9c (65.0%), C16 : 0 (17.8%), and summed feature 8 (10.2 %; comprising C18 : 1ω7c, and/or C18 : 1ω6c). The DNA G+C content of the strains is 34.2 mol%. On the basis of data presented here, strain c10Ua161MT represents a novel species of the genus Lactobacillus , for which the name Lactobacillus mulieris sp. nov. is proposed. The type strain is c10Ua161MT (=CECT 9755T=DSM 108704T).

2020Journal article

Restricted access

Identification and quantification of stilbenes (piceatannol and resveratrol) in passiflora edulis by-products

Publication . Krambeck, Karolline; Oliveira, Ana; Santos, Delfim; Pintado, Maria Manuela; Baptista Silva, João Baptista; Lobo, José Manuel Sousa; Amaral, Maria Helena

Recently, studies on the by-products from the food industry, such as passion fruit seeds, have significantly increased, as these can have an added value, due to their properties, such as potential antioxidant activity. This study was conducted to determine the presence of piceatannol and resveratrol in various extracts of passion fruit (Passiflora edulis) seeds from Madeira Island and a commercial passion fruit oil was used as reference. The commercial oil and the extracts that were obtained by traditional Soxhlet method with ethanol and acetone did not reveal the presence of the two stilbenes, piceatannol and resveratrol. However, the extracts that were obtained by the ultrasound method showed significant amounts of piceatannol and resveratrol when compared with the commercial oil. The presence of these compounds indicates that this oil could have potential application in cosmetic and pharmaceutical industries, due to their proven antioxidant and anti-aging properties.

2020Journal article

Open access