OpenMicroBio: A framework for computational simulation of cellular communities during BioProcess Engineering

Funder

Organizational Unit

Publications

Saccharomyces cerevisiae oxidative response evaluation by cyclic voltammetry and gas chromatography−mass spectrometry

Publication . Castro, Cristiana C.; Gunning, Caitriona; Oliveira, Carla M.; Couto, José A.; Teixeira, José A.; Martins, Rui C.; Ferreira, António C. Silva

This study is focused on the evaluation of the impact of Saccharomyces cerevisiae metabolism in the profile of compounds with antioxidant capacity in a synthetic wine during fermentation. A bioanalytical pipeline, which allows for biological systems fingerprinting and sample classification by combining electrochemical features with biochemical background, is proposed. To achieve this objective, alcoholic fermentations of a minimal medium supplemented with phenolic acids were evaluated daily during 11 days, for electrochemical profile, phenolic acids, and the volatile fermentation fraction, using cyclic voltametry, high-performance liquid chromatography−diode array detection, and headspace/solid-phase microextraction/gas chromatography−mass spectrometry (target and nontarget approaches), respectively. It was found that acetic acid, 2- phenylethanol, and isoamyl acetate are compounds with a significative contribution for samples metabolic variability, and the electrochemical features demonstrated redox-potential changes throughout the alcoholic fermentations, showing at the end a similar pattern to normal wines. Moreover, S. cerevisiae had the capacity of producing chlorogenic acid in the supplemented medium fermentation from simple precursors present in the minimal medium.

2012Journal article

Restricted access

Application of a high-throughput process analytical technology metabolomics pipeline to Port wine forced ageing process

Publication . Castro, Cristiana C.; Martins, R.C.; Teixeira, José A.; Ferreira, António C. Silva

Metabolomics aims at gathering the maximum amount of metabolic information for a total interpretation of biological systems. A process analytical technology pipeline, combining gas chromatography–mass spectrometry data preprocessing with multivariate analysis, was applied to a Port wine ‘‘forced ageing’’ process under different oxygen saturation regimes at 60 C. It was found that extreme ‘‘forced ageing’’ conditions promote the occurrence of undesirable chemical reactions by production of dioxane and dioxolane isomers, furfural and 5-hydroxymethylfurfural, which affect the quality of the final product through the degradation of the wine aromatic profile, colour and taste. Also, were found high kinetical correlations between these key metabolites with benzaldehyde, sotolon, and many other metabolites that contribute for the final aromatic profile of the Port wine. The use of the kinetical correlations in time-dependent processes as wine ageing can further contribute to biological or chemical systems monitoring, new biomarkers discovery and metabolic network investigations.

2014Journal article

Restricted access