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Research Project
DEVELOPMENT AND CHARACTERIZATION OF FUNCTIONAL INGREDIENTS THROUGH VALORIZATION OF SPENT BREWER YEAST: PEPTIDE CONCENTRATES AND β-GLUCANS WITH BIOLOGICAL ACTIVITIES
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Antiulcer and antiproliferative properties of spent brewer's yeast peptide extracts for incorporation into foods
Publication . Amorim, Maria M.; Pereira, Joana O.; Monteiro, Karin M.; Ruiz, Ana L.; Carvalho, João E.; Pinheiro, Hélder; Pintado, M. E.
The main objective was to study the antiulcer and antiproliferative potential of yeast peptide extract for further incorporation into functional foods. Peptide concentrates were obtained by hydrolysis of spent brewer's yeast proteins followed by a filtration process. In order to prove the possible protection of gastric mucosa, an animal model with ulcerative lesions caused by oral administration of absolute ethanol was used. The peptide fraction <3 kDa was able to reduce gastric injuries to significant levels (p < 0.001) and the effective dose (DE50) was 816 mg per kg bw. The cytoprotective effect appears to depend on a prostaglandin-mediated mechanism and also on a nonspecific mechanism. The antiproliferative activity of the extract in nine different human tumoral cell lines was tested. The results exhibited a promising antiproliferative activity against the cell line K-562 (leukemia). The results suggest that a new peptide extract can be used to develop new value-added functional food products, although further studies are required.
Nutritional ingredients from spent brewer's yeast obtained by hydrolysis and selective membrane filtration integrated in a pilot process
Publication . Amorim, Manuela; Pereira, Joana O.; Gomes, David; Pereira, Carlos Dias; Pinheiro, Hélder; Pintado, M. E.
Spent brewer's yeast is a natural surplus from brewing industry. In order to up-grade this by-product, isolation of compounds has been tentatively assessed. The main objective of this work focuses on the use of ultrafiltration and nanofiltration pilot system for recovering cell compounds. Initially, yeast was autolyzed and ultrafiltered with a 10 kDa cut-off, and the two fractions obtained were hydrolyzed with Cynara cardunculus extract and nanofiltered with 3 kDa cut-off. Four fractions with different molecular weights were obtained, with protein and sugar contents ranging between 30–69% and 20–48%, respectively. Sodium and potassium were the major minerals present, whereas glutamine, glutamic acid and alanine, the most representative free amino acids. Peptide profile showed peptides with hydrophilic and hydrophobic characteristics, usually associated with biological activities, including antihypertensive and antioxidant. Thus, based on their compositions, all fractions show technological and biological potential, and can be used as nutritional ingredients in food and feed.
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Funding agency
Fundação para a Ciência e a Tecnologia
Funding programme
FARH
Funding Award Number
SFRH/BD/81901/2011