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- Effects of encapsulation on the viability of probiotic strains exposed to lethal conditionsPublication . Borges, Sandra; Barbosa, Joana; Camilo, Rute; Carvalheira, Ana; Silva, Joana; Sousa, Sérgio; Gomes, Ana M.; Pintado, Maria M.; Silva, José P.; Costa, Paulo; Amaral, Maria H.; Teixeira, Paula; Freitas, Ana C.The effect of microencapsulation on the viability of Lactobacillus casei, L. paracasei, L. acidophilus Ki and Bifidobacterium animalis BB-12 during exposure to lethal conditions (25% NaCl, pH 3.0 and 55–60 ºC) was evaluated. Results demonstrated that survival of probiotic strains to the imposed lethal stress conditions was strain dependent. With the exception of exposure to 25% (w ⁄ v) NaCl, L. acidophilus Ki (free and encapsulated cells) demonstrated the highest survival rates through exposure to lethal conditions of temperature and pH. For this probiotic strain exposed to heat, microencapsulated cells expressed a higher heat tolerance at 55 ºC than free cells. For the other tested bacteria, in general, encapsulation had no positive effect on survival through the tested lethal conditions.