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- Impact of enzyme- and ultrasound-assisted extraction methods on biological properties of red, brown, and green seaweeds from the Central West Coast of PortugalPublication . Rodrigues, Dina; Sousa, Sérgio; Silva, Aline; Amorim, Manuela; Pereira, Leonel; Rocha-Santos, Teresa A.P.; Gomes, Ana M. P.; Duarte, Armando C.; Freitas, Ana CristinaSeaweeds are an excellent source of bioactive compounds, and therefore the use of sustainable and food compatible extraction methods such as enzyme-assisted (EAE) and ultrasound-assisted extraction were applied on Sargassum muticum, Osmundea pinnatifida, and Codium tomentosum. Extracts were evaluated for proximate characterization and biological properties. Higher extraction yields were observed for C. tomentosum EAE (48-62%; p < 0.05 for Cellulase and Viscozyme), followed by O. pinnatifida (49-55%; p < 0.05 except Alcalase) and S. muticum (26-31%; p < 0.05). S. muticum extracts presented the highest nitrogen (25 +/- 2 mg/g(lyoph) (extract)) and total phenolics (261 +/- 37 mu g(catechol) (equiv)/gl(yoph) (extract)) contents, whereas higher sugars (78 +/- 14 mg(glucose) (equiv)/g(lyoph) (extract)) including sulfated polysaccharide (44 +/- 8 mg(Na2SO4) (acid)/g(lyoph) (extract)) contents characterized O. pinnatifida extracts. A higher effect on hydroxyl-radical scavenging activity (35-50%) was observed for all extracts, whereas S. muticum Alcalase and C. tomentosum Cellulase extracts exhibited higher prebiotic activity than fructooligosaccharides. O. pinnatifida and C. tomentosum EAE showed inhibitory potential against alpha-glucosidase (38-49%).