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Microsequential injection lab-on-valve system for the spectrophotometric bi-parametric determination of iron and copper in natural waters
Publication . González, Alba; Mesquita, Raquel B. R.; Avivar, Jessica; Moniz, Tânia; Rangel, Maria; Cerdà, Víctor; Rangel, António O. S. S.
The determination of iron and copper exploiting a microsequential injection lab-on-valve system with online spectrophotometric detection is described. A new, environmental friendly 3-hydroxy-4-pyridinone chelator, functionalized with a polyethylene glycol chain (MRB12) to improve water solubility, was used for iron determination. For copper determination, 1-(2-pyridylazo)-2-naphthol (PAN) was used. Different parameters affecting the formation of the complexes were studied, namely sample volume, reagent concentration, and buffer composition and concentration. The optimized conditions, 150 mu L of sample volume and 250 mg L-1 of MRB12 for iron determination, and 200 mu L of sample and 120 mg L-1 of PAN for copper determination, enabled an LOD of 15 and 18 mu g L-1 for iron and copper, respectively. The robustness of the developed procedure was assessed by the calculation of the relative standard deviation (RSD), 5% for iron determination and 2% for copper determination. The accuracy of the method was assessed comparing the results with two certified samples (RD < 7.5%) and calculating recovery percentages with five river water samples (average < 107%).
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