CBQF - Contribuições em Revistas Científicas / Contribution to Journals
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Browsing CBQF - Contribuições em Revistas Científicas / Contribution to Journals by Sustainable Development Goals (SDG) "14:Proteger a Vida Marinha"
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- Exploring the biodegradation of PET in mangrove soil and its intermediates by enriched bacterial consortiaPublication . Saidu, Muhammad Bashir; Moreira, Irina S.; Amorim, Catarina L.; Wu, Rongben; Ho, Yuen-Wa; Fang, James Kar-Hei; Castro, Paula M. L.; Gonçalves, DavidThe biodegradation of Polyethylene terephthalate (PET) is important due to the environmental impact of plastic waste. This study investigates the degradation of PET films in soil microcosms, with and without mangrove plants, and with mangrove plants bioaugmented with a bacterial consortium (Bacillus sp.- GPB12 and Enterococcus sp.- WTP31B-5) while following the evolution of soil microcosm microbiome. The ability of bacterial consortia retrieved from soil microcosms of each tested condition to degrade PET intermediates - bis(2-hydroxyethyl) terephthalate (BHET), terephthalic acid (TPA), and monoethylene glycol (MEG) was also assessed. In the microcosms’ assays with mangrove plants, variations in functional groups and surface morphology detected by FTIR and SEM analysis indicated PET degradation. Soil microcosms microbiome evolved differently according to the conditions imposed, with dominance of phylum Proteobacteria in all final microcosms. After 270 days, bacterial consortia retrieved from all soil microcosms revealed to be able to completely degrade TPA within three days. MEG degradation reached ca. 84% using the consortium retrieved from the microcosm with bioaugmented mangrove plants. BHETdegradation was ca. 96% with the consortium obtained from the microcosm with non-bioaugmented mangrove plants. These intermediates are key molecules in PET degradation pathways; thus, their degradation is an indicator of biodegradation potential. To the best of authors’ knowledge, this is the first report on biodegradation of PET, BHET, TPA, and MEG by microbial community from mangrove soil, providing insights into key taxa involved in PET degradation. These findings can pave a way to develop bioremediation strategies and more efficient waste management solutions.
- Inclusion of pineapple by-products as natural antioxidant sources in diets for European sea bass (Dicentrarchus labrax)Publication . Pereira, Ricardo; Basto, Ana; Pintado, Manuela; Valente, Luisa M. P.; Velasco, CristinaThis study investigates the effects of pineapple by-products on feed preservation during storage at two different temperatures (25 °C and 4 °C) and on European sea bass (Dicentrarchus labrax) stress resistance. Four isoproteic, isolipidic, and isoenergetic diets were manufactured: CTRL—negative control, commercial diet without added antioxidants; VITE—positive control, CTRL diet with 100 mg kg−1 of vitamin E; and P2 and S2—VITE diet with 2% pineapple peel or stem flour, respectively. The fish (13.5 ± 0.8 g) were split into four replicate groups per diet and fed ad libitum for 12 weeks, after which they were subjected to a stress challenge of air exposure (1 min) followed by confinement (5 min, 100 kg m−3). Despite storage time lowering the antioxidant properties of all diets, P2 and S2 showed increased antioxidant capacity (DPPH•, ABTS•+, and ORAC) before and after storage. The diets were well accepted by the fish, and the VITE-fed fish showed significantly lower lipid peroxidation values in the liver and muscle compared to all remaining diets. However, pineapple by-product inclusion did not result in increased fish stress resistance. Further optimization is required for the successful use of pineapple by-products as natural antioxidants in aquafeeds.