Browsing by Issue Date, starting with "2022-08-30"
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- Parody, irony and satire: literary approaches to rewriting and subverting realityPublication . Martins, José Cândido de Oliveira; Kołakowski, Marcin
- Valorization of brewery waste through polyhydroxyalkanoates production supported by a metabolic specialized microbiomePublication . Carvalheira, Mónica; Amorim, Catarina L.; Oliveira, Ana Catarina; Guarda, Eliana C.; Costa, Eunice; Teixeira, Margarida Ribau; Castro, Paula M. L.; Duque, Anouk F.; Reis, Maria A. M.Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the Meganema, Carnobacterium, Leucobacter, and Paracocccus genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 CmmolPHA/CmmolVFA in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products.
- Quality control of drinking water in the city of Ilave, Region of Puno, PeruPublication . Ferro, Pompeyo; Rossel-Bernedo, Luis Jhordan; Ferró-Gonzáles, Ana Lucia; Vaz-Moreira, IvoneThe region of Puno, in Peru, is described as a region with some health conditions that may be associated with the water quality, such as a high index of anemia or cases of acute diarrhea in children. This study aimed at monitoring the drinking water quality of the city of Ilave, in Peru, and determining possible correlations between physical-chemical and microbiological parameters, and the water distribution conditions, such as the period of water availability. Physical-chemical parameters (turbidity, residual chlorine, temperature, conductivity, and pH), microbiological parameters (presence of coliforms), and heavy metals (Zn, Mn, Ni, Fe, and Cu) were determined. All the parameters quantified were within the maximum permissible limits according to Peruvian regulations, except for residual chlorine, which was, for all the treated water samples, below the recommended value of 0.5 mg/L. Coliforms that should be absent from drinking water were detected in all the household samples. These results demonstrate the need for the inclusion of additional steps of re-chlorination along the distribution system to guarantee the maintenance of residual levels of chlorine that assure the microbiological quality of water. The quality of the drinking water was not observed to correlate with the period of water availability.