Percorrer por autor "Vasconcelos, Andreanne Gomes"
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- Acetylated cashew gum and fucan for incorporation of lycopene rich extract from red guava (Psidium guajava L.) in nanostructured systems: antioxidant and antitumor capacityPublication . Andrades, Eryka Oliveira de; Costa, João Marcos Antônio Rodrigues da; Neto, Francisco Edmar Moreira de Lima; Araujo, Alyne Rodrigues de; Ribeiro, Fabio de Oliveira Silva; Vasconcelos, Andreanne Gomes; Oliveira, Antônia Carla de Jesus; Sobrinho, José Lamartine Soares; Almeida, Miguel Peixoto de; Carvalho, Ana P.; Dias, Jhones Nascimento; Silva, Ingrid Gracielle Martins; Albuquerque, Patrícia; Pereira, Ildinete Silva; Rabello, Doralina do Amaral; Amorim, Adriany das Graças Nascimento; Leite, José Roberto de Souza de Almeida; Silva, Durcilene Alves daIndustrial application of lycopene is limited due to its chemical instability and low bioavailability. This study proposes the development of fucan-coated acetylated cashew gum nanoparticles (NFGa) and acetylated cashew gum nanoparticles (NGa) for incorporation of the lycopene-rich extract from red guava (LEG). Size, polydispersity, zeta potential, nanoparticles concentration, encapsulation efficiency, transmission electron microscopy (TEM) and atomic force microscopy (AFM) were used to characterize nanoparticles. The antioxidant activity was determinated and cell viability was evaluated in the human breast cancer cells (MCF-7) and human keratinocytes (HaCaT) by MTT assay. The toxic effect was evaluated by hemolysis test and by Galleria mellonella model. NFGa showed higher stability than NGa, having a size of 162.10 ± 3.21 nm, polydispersity of 0.348 ± 0.019, zeta potential -30.70 ± 0.53 mV, concentration of 6.4 × 109 nanoparticles/mL and 60% LEG encapsulation. Microscopic analysis revealed a spherical and smooth shape of NFGa. NFGa showed antioxidant capacity by ABTS method and ORAC assay. The NFGa presented significant cytotoxicity against MCF-7 from the lowest concentration tested (6.25-200 μg/mL) and did not affect the cell viability of the HaCaT. NFGa showed non-toxic effect in the in vitro and in vivo models. Therefore, NFGa may have a promising application in LEG stabilization for antioxidant and antitumor purposes.
- Bio-availability, anticancer potential, and chemical data of lycopene: an overview and technological prospectingPublication . Amorim, Adriany das Graças Nascimento; Vasconcelos, Andreanne Gomes; Souza, Jessica; Oliveira, Ana; Gullón, Beatriz; Leite, José Roberto de Souza de Almeida; Pintado, ManuelaThe purpose of this review was to collect relevant chemical data about lycopene and its isomers, which can be extracted using different non-polar or polar aprotic solvents by SC-CO2 or biosynthesis as a friendly technique. Lycopene and other carotenoids can be identified and quantified by UV–Vis and HPLC using a C18 or C30 column, while their characterization is possible by UV–Vis, Fluorescence, FTIR, MS, NMR, and DSC assays. Among these techniques, the last four can compare lycopene isomers and identify cis or all-trans-lycopene. FTIR, MS, and NMR techniques are more suitable for the verification of the purity of lycopene extracts due to the signal complexity generated for each isomer, which enables identification by subtle differences. Additionally, some biological activities of lycopene isolated from red vegetables have already been confirmed, such as anti-inflammatory, antioxidant, and cytotoxic activity against cancer cells, probably by activating several pathways. The encapsulation of lycopene in nanoparticles demonstrated an improvement in oral delivery, and ex vivo assessments determined that these nanoparticles had better permeation and low cytotoxicity against human cells with enhanced permeation. These data suggest that lycopene has the potential to be applied in the food and pharmaceutical industries, as well as in cosmetic products.