Percorrer por autor "Sousa, Teresa"
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- Biohybrid dressings: integrating silk fibroin textiles with decellularized biological tissue for wound healingPublication . Sousa, Teresa; Vale, Inês; Rosadas, Marta; Silva, Inês V.; Ribeiro, Viviana P.; Oliveira, Ana L.Burns affect 11 million people globally each year with 180,000 associated fatalities. This study proposes a multilayer hybrid dressing combining a silk fibroin (SF) textile with decellularized porcine small intestine (dPSI) capable of simultaneously provide wound protection and tissue regeneration. A silk sericin (SS) hydrogel was embedded into the decellularized tissue ensuring the integration with the textile layer while promoting anti-inflammatory benefits, enhancing the hybrid dressing’s biological performance.
- Biohybrid solutions for burn care: merging silk medical textiles with decellularized matricesPublication . Sousa, Teresa; Vale, Inês; Rosadas, Marta; Silva, Inês V.; Ribeiro, Viviana P.; Oliveira, Ana L.
- Biohybrid solutions for burn care: merging silk medical textiles with decellularized matricesPublication . Sousa, Teresa; Vale, Inês; Rosadas, Marta; Silva, Inês V.; Ribeiro, Viviana P.; Oliveira, Ana L.
- Cutting-edge hybrid dressings: combining silk medical textiles and decellularized biological tissue for advanced burn wound carePublication . Sousa, Teresa; Vale, Inês; Rosadas, Marta; Silva, Inês V.; Ribeiro, Viviana P.; Oliveira, Ana L.Aim: Burns affect 11 million people globally each year, with 180,000 fatalities [1]. This study investigates a multilayer burn dressing combining silk fibroin (SF) fabric with highly- preserved decellularized porcine small intestine (dPSI) to support tissue regeneration and wound integration [2]. A silk sericin (SS) hydrogel is included as interface for structural integrity and anti-inflammatory benefits, enhancing the hybrid dressing’s biological performance. Methods: An innovative decellularization protocol was proposed to obtain dPSI, maintaining submucosa, serosa, and muscle layers, using cycles of decellularizing agents (SDS, SDC, DMSO), washing agents (upH₂O, PBS), and sterilization (PAA/ethanol). The serosa and submucosa of dPSI were integrated with SF-based textiles using HRP- crosslinked SS hydrogels. Decellularization and integrity were assessed via DNA quantification and histology, while hybrid dressings’ morphology and mechanics were evaluated by SEM and tensile tests. Degradation profile was tested in simulated wound fluid, and biological performance was assessed by culturing human dermal fibroblasts (hDFs) on the submucosa layer up to 10 days. Results: The dPSI was successfully achieved (<50 ng/mg dsDNA). SEM images confirmed the full integration of the dPSI with SF-based textiles, especially when serosa faced the textile. The presence of the textile structure resulted in an enhancement of the mechanical strength. dPSI was able to degrade first in the multilayer dressing, and hDFs adhered and proliferated on the submucosa over 10 days, supporting hybrid structural integrity. Conclusions: This study is pioneer in confirming promising results for the first multilayer hybrid dressing combining medical textiles and dPSI for burn wound applications.
- Development of a decellularized extracelular matrix from porcine aorta for heart valve applications in the Ross procedurePublication . Reis, Mariana S.; Rosadas, Marta; Ho, Chou I.; Sousa, Teresa; Pazmino, Carlos; Costa, João; Vervenne, Thibault; Oliveira, Ana L.; Ribeiro, Viviana P.; Mignon, ArnCardiovascular diseases are the leading cause of adult mortality worldwide, according to the World Health Organization [1]. An important surgical approach for treating diseased aortic valves is the Ross procedure, in whic the affected aortic valve is replaced with an autograft from the patient’s own pulmonary valve.
- Diagnosis and laboratory follow-up of patients with multiple myeloma: guidelines from the Portuguese multiple myeloma groupPublication . Pires, Ana Marta; Barreto, João Pedro; Caetano, Joana; Soares, Maria José; Geraldes, Catarina; Fernandes, Bruno; Coucelo, Margarida; Chacim, Sérgio; Coelho, Henrique; Correia, Cecília; Cruz, Ana Paula; Cunha, Manuel; Cunha, Maria Rosário; Cunha, Nuno; Ferraz, Patrícia; Freitas, José Guilherme; Henrique, Rui; Lisboa, Susana; Lúcio, Paulo; Paiva, Artur; Pedrosa, Cláudia; Ramos, Inês; Sarmento, Ana Bela; Seabra, Patrícia; Sevilha, Joana; Sousa, Maria José Rego de; Sousa, Sara; Sousa, Teresa; Tavares, Márcio; Trigo, Fernanda; Bergantim, Rui; Roque, Adriana; João, CristinaMultiple myeloma is a neoplasm of plasma cells that in most cases is associated with the secretion of monoclonal immunoglobulins and can involve multiple organs. Its timely diagnosis is essential to limit or avoid irreversible damage and dysfunction of target organs. Appropriate initial stratification of patients allows for optimization in the selection and sequence of therapy, as well as proper follow-up during treatment and monitoring, impacting survival. These laboratory guidelines from the Portuguese Multiple Myeloma Group provide recommendations for the diagnosis and laboratory follow-up of patients with multiple myeloma. The follow-up and diagnosis of patients with other clinically significant monoclonal gammopathies were not included in this text. This article was based on international guidelines, scientific publications, and the experience of a panel of specialists in clinical and laboratory fields dedicated to the study and treatment of multiple myeloma.
- Exploring new biobased material sources as platforms to advance skin wound healing and regenerationPublication . Ribeiro, Viviana; Bernardes, Beatriz G.; Duarte, Marta; Rosadas, Marta; Sousa, Teresa; Sousa, Alda; Serra, Julia; García-González, Carlos; Oliveira, Ana L.Chronic wounds are one of the major therapeutic and healthcare challenges affecting the population globally. One of the research interests of the Biomaterials and Biomedical Technology Lab (BBT Lab) is to explore the potential of biobased material platforms to advance skin wound healing and regeneration solutions. From the use of natural based biopolymers such as silk fibroin (SF) or sulfated exopolysaccharides (EPS), to the processing of more complex matrices such as the extracellular matrices, the group has been collaborating with some strategic partners in IBEROS+ to process, functionalize and characterize the materials for their physicochemical properties, structural adaptability, biocompatibility and bioactivity. SF microparticulate aerogels loaded with adenosine have been developed via supercritical fluid technology in collaboration with the University of Santiago de Compostela. These particles exhibit a high porosity, biocompatibility, and positive interactions with skin cells towards regeneration, highlighting their promise in wound healing. A new Exopolysaccharide (EPS) produced by Porphyridium cruentum microalgae was developed as a novel biomaterial platform, offering bioactive properties, high molecular weight, thermal stability, and cytocompatibility for complex wound healing. An extensive characterization is ongoing, with contribution of the University of Vigo. For extensive burn wounds, where autologous grafts are impractical, skin xenografts may provide a viable alternative, mostly if depleted from its immunogenic load. To achieve this, our group has developed and optimized methods for obtaining highly-preserved animal- origin decellularized tissues for human skin healing and regeneration. An important example is the valorization of rabbit skin, a valuable agro-food by-product that exceeds 5000 skins/day only in Europe. Our group has recently developed decellularized rabbit dermal matrices with preserved microarchitecture and human-like biochemical properties and expects to continue further developments in collaboration with the IBEROS+ consortium.
- Exploring rabbit skin as a novel decellularized dermal substitute for burn wound healingPublication . Rosadas, Marta; Sousa, Teresa; Silva, Inês V.; Sousa, Alda; Ribeiro, Viviana P.; Oliveira, Ana L.Aim: Burn wounds represent a significant medical challenge. Autografts remain the standard treatment, however, are unsuitable for deep or extensive burns(1). Decellularized skin xenografts offer a viable alternative, having significantly reduced immunogenicity, still there is currently no skin-derived decellularized xenografic matrix due to its low resemblance to human skin (2)(3). This study proposes decellularized rabbit skin as a viable mimetic alternative. We describe a novel protocol for valorizing an agro-food by-product, exceeding 5000 skins/day, into highly preserved decellularized rabbit dermal matrices (HP-dRDMs) for burn wound treatment and skin regeneration. Method: Rabbit skin by-products were processed at Cortadoria Nacional de Pêlo S.A., following pioneer methodologies obtaining rabbit dermis for tannery. Decellularization agents (EDTA, SDS, and SDC) were further applied with varying exposure times. Decellularization efficiency was confirmed through DNA quantification, and extracellular matrix (ECM) preservation evaluated by ECM components quantification, morphological analysis, swelling properties, and mechanical behavior. Biocompatibility was assessed by in vitro culturing human dermal fibroblasts (hDFs) up to 10 days. Results/Discussion: The results show pH influences the collagen matrix conformation and swelling capability. Differences in the dermis’ topography were observed depending on the decellularization agents and exposure time. Mechanical analysis revealed similar performance to human skin, especially with the SDC protocol. DNA quantification confirmed successful decellularization. In vitro, hDFs adhered, spread, and proliferate within the HP-dRDM over the 10 days of culture. Conclusion: This study demonstrates a successful protocol for rabbit dermis decellularization, preserving ECM components and yielding high-quality matrices with biological, structural, and biomechanical properties to support skin regeneration in burn wounds.
- Exploring rabbit skin as a novel decellularized dermal substitute for burn wound healingPublication . Rosadas, Marta; Sousa, Teresa; Silva, Inês V.; Sousa, Alda; Ribeiro, Viviana P.; Oliveira, Ana L.Introduction: Burn wounds remain a significant challenge in medical care, requiring effective wound coverage to restore the skin barrier and promote healing or support skin reconstruction. The use of autologous grafts as substitutes is still the standard treatment, however, it presents limitations for deep and extensive burns (1). Decellularized skin allografts and xenografts have emerged as suitable options, using decellularization to remove the immunogenic material present in the tissue while preserving the ECM components and interesting biomolecules (3). Considering that xenografts source availability is significantly higher and free of ethical concerns, this study describes for the first time a protocol for decellularizing rabbit dermis, leveraging a valuable agro-food by-product that exceeds 5000 skins/day at the world-leading company Cortadoria Nacional de Pêlo, and studies its potential for skin regeneration. Conclusions: A minimally invasive protocol was successfully optimized for obtaining dRDMs, yielding matrices with DNA content below 50 ng dsDNA/mg, meeting decellularization standards. The preserved mechanical properties, comparable to controls, indicate structural integrity. The GAGs quantification showed that this ECM componet is in a range that can influence cells. Furthermore, in vitro studies confirmed the cytocompatibility of dRDMs, highlighting their potential for biomedical applications.
- Is decellularized rabbit dermis a viable option for skin wound healing and regeneration?Publication . Rosadas, Marta; Sousa, Teresa; Sousa, Alda; Ribeiro, Viviana P.; Oliveira, Ana L.Burn wounds remain a significant challenge in medical care, requiring effective wound coverage to restore the skin barrier and promote healing or support skin reconstruction. The use of autologous grafts as substitutes is still the standard treatment, however, it is not suitable for deep and extensive burns (1). Decellularized skin allografts and xenografts have emerged as suitable options, using decellularization to remove the immunogenic material present in the tissue while preserving the ECM components and interesting biomolecules (3). Considering that xenografts source availability is significantly higher and free of ethical concerns, this study describes for the first time a protocol for decellularizing rabbit dermis, leveraging a valuable agro-food by-product which exceeds 5000 skins/day at the world-leading company Cortadoria Nacional de Pêlo, and studies its potential for skin regeneration.