Browsing by Author "Silva, Solranny Carla Cavalcante Costa"
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- Chitosan/collagen biomembrane loaded with 2,3-dihydrobenzofuran for the treatment of cutaneous LeishmaniasisPublication . Braz, Elton Marks Araujo; Silva, Solranny Carla Cavalcante Costa; Alves, Michel Muálem Moraes; Carvalho, Fernando Aécio Amorim; Magalhães, Rui; Osajima, Josy Anteveli; Silva, Durcilene Alves; Oliveira, Ana Leite; Muniz, Edvani Curti; Silva-Filho, Edson CavalcantiIn this work, chitosan/collagen-based membranes loaded with 2,3-dihydrobenzofuran (2,3-DHB) were developed through a simple solvent-casting procedure for use in the treatment of cutaneous Leishmaniasis. The obtained membranes were characterized by elemental analysis, FTIR, TG, DSC, and XRD. Porosity, swelling, mechanical properties, hydrophilicity, and antioxidant activity were analyzed. In addition, assessment to the biocompatibility, through fibroblasts/keratinocytes and in vitro wound healing essays were performed. The obtained results show that the new 2,3-DHB loaded chitosan/collagen membrane presented high porosity and swelling capacity as well as maximum strength, hydrophilicity, and antioxidant activity higher in relation to the control. The tests of antileishmanial activity and the AFM images demonstrate great efficacy of inhibition growth of the parasite, superior to those from the standard therapeutic agent that is currently used: Amphotericin B. The new membranes are biocompatible and stimulated the proliferation of keratinocytes. SEM images clearly demonstrate that fibroblasts were able to adhere, maintained their characteristic morphology. The healing test evidenced that the membranes have adequate environment for promoting cell proliferation and growth. As the conventional treatments often use drugs with high toxicity, the as-developed new membranes proved to be excellent candidate to treat cutaneous Leishmaniasis and can be clearly indicated for further advanced studies in vivo.
- Modified chicha gum by acetylation for antimicrobial and antiparasitic applications: characterization and biological propertiesPublication . Braz, Elton Marks Araujo; Silva, Solranny Carla Cavalcante Costa; Brito, Carla Adriana Rodrigues Sousa; Carvalho, Fernando Aécio Amorim; Alves, Michel Muálem Moraes; Barreto, Humberto Medeiros; Silva, Durcilene Alves; Magalhães, Rui; Oliveira, Ana Leite; Silva-Filho, Edson C.It was developed a material to act as an antimicrobial and antiparasitic agent through a modification reaction in the gum structure extracted from the plant Sterculia striata. This material was characterized, the oxidant activity was evaluated and the antimicrobial activity against Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Salmonella Typhimurium and Klebsiella pneumoniae was investigated, in addition to the effect against Leishmania amazonensis, testing its acute toxicity and its cytotoxicity in human cells. Characterization techniques proved the success of chemical modification. The modification led to an increase in antioxidant activity, with excellent antibacterial activity, reaching almost 100% inhibition for P. aeruginosa and S. Typhimurium, and inhibitory effect above 70% against L. amazonensis, with an affinity far superior to the parasite than macrophages. The derivative showed no acute toxicity, it was non-hemolytic, increased cell viability in macrophages and fibroblasts, and stimulated cell proliferation of keratinocytes, thus being a strong candidate to be used as an antimicrobial and antiparasitic agent in biomedical applications.
- Phthalic anhydride esterified chicha gum: characterization and antibacterial activityPublication . Silva, Solranny Carla Cavalcante Costa; Braz, Elton Marks Araujo; Brito, Carla Adriana Rodrigues Sousa; Alves, Michel Muálem Moraes; Carvalho, Fernando Aécio Amorim; Barreto, Humberto Medeiros; Oliveira, Ana Leite; Silva, Durcilene Alves; Silva-Filho, Edson C.The objective of this research was to modify chicha gum with phthalic anhydride to obtain a new biologically active material. The chemical modification of the gum structure was proven through FTIR, elemental analysis, XRD, TG, and DSC. The derived materials demonstrated excellent inhibitory effect against P. aeruginosa and K. pneumoniae species (rating 100% inhibition) and could also inhibit Escherichia coli growth. The best antimicrobial activity observed for the derivatives suggests that chicha gum hydrophobization due to the addition of phthalic groups improved the interaction of these derivatives with bacterial cell wall components. On the other hand, the derivatives increased CC50 in macrophages but did not present acute toxicity or hemolytic activity, indicating that they are promising for use in prophylaxis or treatment of infections caused by Gram-negative bacteria.