Browsing by Author "Rodrigues, Dina M."
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- Effect of probiotic co-cultures on physico-chemical and biochemical properties of small ruminants’ fermented milkPublication . Pinto, Jorge M. S.; Sousa, Sérgio; Rodrigues, Dina M.; Malcata, Francisco X.; Duarte, Armando C.; Rocha-Santos, Teresa A. P.; Freitas, Ana C.; Gomes, Ana M.Small ruminants' fermented probiotic milk is an alternative to fermented cows' milk, especially because of the monounsaturated/polyunsaturated fatty acid profiles. The technological and biochemical potential of Bifidobacterium and Lactobacillus co-cultures, with or without inulin, on goats' and ewes' milk was assessed. Microbial stability, lactose consumption, organic acid production, proteolytic parameters and conjugated linoleic acid (CLA) production in situ, were followed in ewes' and goats’ fermented milk (EFM and GFM, respectively) over 21 days at 4 °C; technological feasibility for probiotic fermented milk production was shown. In EFM, all co-cultures presented high viable cell numbers (>7.0 log cfu mL−1) throughout storage, presenting faster acidification capacities and higher CLA isomer levels than in GFM. Inulin had no impact on probiotic growth, yet contributed to storage stability. CLA isomers and proteolysis indices were co-culture dependent traits: for example, co-culture of Bifidobacterium animalis B94 with Lactobacillus acidophilus L10 registered the best CLA-production in GFM.
- Optical fibre-based methodology for screening the effect of probiotic bacteria on conjugated linoleic acid (CLA) in curdled milkPublication . Silva, Lurdes I. B.; Rodrigues, Dina M.; Freitas, Ana C.; Gomes, Ana M.; Rocha-Santos, Teresa A. P; Pereira, M. E.; Duarte, A. C.A methodology based on optical fibre (OF) detection was developed for screening the potential of CLA production by Lactobacillus casei-01, Lactobacillus acidophilus La-5 and Bifidobacterium lactis B94 in probiotic curdled milk. The OF based methodology was validated by comparison with an analytical method based on gas chromatography-mass spectrometry (GC-MS) and it showed comparable linearity (between 5 and 130 mu g), accuracy and detection limits, which ranged from 1.92 to 2.56 mu g for CLA methyl ester and oleic acid methyl ester, respectively. Furthermore, the proposed OF based methodology was an advantageous analytical methodology for screening CIA production in probiotic curled milk, due to its compact design and effective cost of analysis. The application of the OF analytical method allowed to detect CLA in the probiotic curdled milk: the production of CLA by probiotic enzymes reached values of 297 +/- 19 mu g g(-1) by 60 days of ripening unfolding this potential functional food as very interesting from a technological point of view.