Percorrer por autor "Rocha, Cristina M. R."
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- Advances in extraction methods to recover added-value compounds from seaweeds: sustainability and functionalityPublication . Matos, Gabriela S.; Pereira, Sara G.; Genisheva, Zlatina A.; Gomes, Ana Maria; Teixeira, José A.; Rocha, Cristina M. R.Seaweeds are a renewable natural source of valuable macro and micronutrients that have attracted the attention of the scientists in the last years. Their medicinal properties were already recognized in the ancient traditional Chinese medicine, but only recently there has been a considerable increase in the study of these organisms in attempts to demonstrate their health benefits. The extraction process and conditions to be used for the obtention of value-added compounds from seaweeds depends mainly on the desired final product. Thermochemical conversion of seaweeds, using high temperatures and solvents (including water), to obtain high-value products with more potential applications continues to be an industrial practice, frequently with adverse impact on the environment and products’ functionality. However more recently, alternative methods and approaches have been suggested, searching not only to improve the process performance, but also to be less harmful for the environment. A biorefinery approach display a valuable idea of solving economic and environmental drawbacks, enabling less residues production close to the much recommended zero waste system. The aim of this work is to report about the new developed methods of seaweeds extractions and the potential application of the components extracted.
- Biological and chemical valorization potential of portuguese seaweeds: Ulva rigida, Gracilaria sp. and Fucus vesiculosusPublication . Matos, Gabriela; Pereira, Sara G.; Teixeira-Guedes, Catarina I.; Gomes, Ana M. P.; Teixeira, José A.; Rocha, Cristina M. R.
- A comprehensive review of fish protein hydrolysates targeting pet food formulationsPublication . Ribeiro, Tânia Bragança; Maia, Margarida R. G.; Fonseca, António J. M.; Marques, Bianca; Caleja, Cristina; Rosa, Ana; Martins, Rui; Almeida, André; Mota, Maria J.; Aires, Tiago; Rocha, Cristina M. R.; Teixeira, José A.; Cabrita, Ana R. J.; Barros, Lillian; Pintado, ManuelaThe fish industry generates significant amounts of fish by- and co-products (FBCPs) annually, projected to reach 160.8 million tonnes by 2030. This growth highlights the urgent need for sustainable FBCP management and an opportunity to improve the sector's environmental sustainability. Fish protein hydrolysates (FBCPHs) and bioactive peptides (BPs) derived from these FBCPs are gaining recognition in the pet food sector for their nutritional and bioactives benefits. FBCPHs, primarily sourced from category 3 by-products unsuitable for human consumption, could significantly enhance the economic viability of both industries. This review analyzes production processes, highlighting the benefits and challenges of enzymatic hydrolysis and reviewed emerging technologies such as subcritical water hydrolysis (SWH), which are promising sustainable alternatives by enhancing extraction efficiency and reducing energy consumption. The review explores FBCPHs' applications in pet food, focusing on beneficial biological activities (e.g. antioxidant, prebiotic, neuroprotective). Findings show FBCPHs have significant potential in pet food formulations, providing palatability, hypoallergenic benefits, and addressing health concerns like gastrointestinal disorders and stress-related behaviors. However, further research is required to optimize production processes, scale industrial application, and ensure regulatory compliance. In conclusion, FBCPHs present a valuable solution for promoting sustainability, improving pet nutrition, and supporting the circular economy.
- Enzymatic approach for the extraction of bioactive fractions from red, green and brown seaweedsPublication . Teixeira-Guedes, Catarina; Gomes-Dias, Joana S.; Cunha, Sara A.; Pintado, Manuela E.; Pereira, Ricardo N.; Teixeira, José A.; Rocha, Cristina M. R.Although several enzymatic approaches have been applied with this intent, the sequential use of enzymes covering both cellulolytic and proteolytic activity has never been performed in seaweeds. Sequential use of these enzymes improved the overall extraction yield by up to 160%, 30% and 80% in the different seaweeds when compared to the control condition (water extraction), use of a carbohydrases’ cocktail alone and use of proteases alone, respectively. Regarding the proximate composition of extracts, it proved to be an efficient approach for the solubilization of carbohydrates (up to 28% in G. vermiculophylla, 66% in P. dioica, 77% in U. rigida and 35% in F. vesiculosus) and protein (up to 55% in G. vermiculophylla, 47% in P. dioica, 52% in U. rigida and 42% in F. vesiculosus). For all biomasses, the combination of enzymes induced a significant increase in antioxidant activity, not only by the increase of phenolic compounds but also by the hydrolysis of protein to peptides. Moreover, extracts from red seaweeds displayed prebiotic activity which can be ascribed to their increased content in oligosaccharides and protein/peptides. Overall, the sequential use of enzymes with different activities demonstrated to be an efficient approach for extracting functional fractions to be used as functional ingredients to improve the nutritional value (e.g. in proteins) and/or to include antioxidant and prebiotic features in the food product.
- Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extractionPublication . Marques, Bianca; Nunes, Rafaela; Araújo-Rodrigues, Helena; Pintado, Manuela; Pereira, Ricardo N.; Teixeira, José A.; Rocha, Cristina M. R.Pork represents a major fraction of the meat consumed worldwide but only 30% of the blood generated in slaughterhouses is re-used as raw material for food and feed. Innovative technologies and efficient processing strategies capable of generating added-value products from it are now attracting attention. In this study, the hydrolysis of porcine coagulated blood using sub-critical solvent extraction was investigated. Biomass was hydrolyzed using different temperatures (120–210 °C), applying only water (sub-critical water; SCW) or water with a low concentration of alkali (0.1 mol L−1 NaOH) and different reaction times (30–90 min). Resultant hydrolysates were analyzed for crude and soluble protein, peptide profile, and bioactivity by combining protein quantification, antioxidant activity, and fast protein liquid chromatography measurements. Results showed that increasing temperature increases the degree of hydrolysis and that the addition of NaOH enhances the solubilization of peptides with high molecular weights. Also, hydrolysates showed interesting antioxidant activity, being 60 min the time of reaction with best antioxidant activity. Nevertheless, using only water (SCW) as solvent, without chemical additives, allows the delivering of interesting protein-based bioactive fractions. Sub-critical solvent treatment of porcine blood resulted in added-value fractions with potential bioactivities through a simple and environmentally friendly process.
- Swine blood hydrolysates as promising ingredients for European seabass (D. labrax) dietsPublication . Resende, Daniela; Costas, Benjamin; Sá, Tiago; Golfetto, Umberto; Machado, Marina; Pereira, Miguel; Pereira, Carlos; Marques, Bianca; Rocha, Cristina M. R.; Pintado, Manuela; Valente, Luísa M. P.In this work, we hypothesised that peptide-rich swine blood hydrolysates (BH) could be beneficial ingredients for European seabass diets, due to their potential antioxidant, mineralbinding, immunomodulatory or antimicrobial activities. Furthermore, this inclusion could address T. maritimum infections that occur in aquaculture farms, with high impact on fish wellbeing and aquaculture production, while promoting circular economy. Three fractions of swine BH were studied: one obtained by autohydrolysis (AH); two obtained enzymatically and submitted either to a micro- (MF) or nanofiltration (NF). Dried hydrolysates were included in isolipidic and isoproteic diets for European seabass: a fishmeal (FM) based diet (positive control, PC), a commercial-based diet where 50% of FM was replaced by vegetable proteins (negative control, NC) and three diets where 3% of each BH was added to the NC. Diets were assigned to triplicate groups of 71 juveniles (initial weight 12.3±1.4 g), fed three times daily ad libitum in a recirculating saltwater system. Growth, nutrient utilisation and whole-body composition were evaluated after 12 weeks. At the end of the trial, ten fish per tank were infected with Tenacibaculum maritimum (3.5×105 cfu/L), in a two-hour water bath, and mortality was assessed for 8 days. Fish fed PC had the highest final weight, followed by NC and NF. NC had a significantly higher weight than the MF and AH groups. Specific growth rate (SGR) and feed conversion ratio (FCR) of fish fed NF showed no significant differences from PC and NC. Fish fed AH also displayed similar SGR to NF and NC but significantly higher than MF. Despite no differences regarding final body composition, lipid and energy retention and gain were lowest in fish fed MF, which also had the lowest condition factor. Protein retention was unaffected by dietary treatments. Regarding the infectious challenge, NF had the lowest mortality rate, differing significantly from the NC and MF groups. Mortality for AH and PC groups did not differ from NF. MF showed the highest mortality. Overall, results indicate that dietary supplementation with NF improves European seabass resistance to T. maritimum without affecting growth rate and can hence become a valuable ingredient for this species.