Browsing by Author "Ribeiro, Carlos F."
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- PVP formulations of bis-cyclometalated iridium(III) complexes bearing β-modified porphyrin ligands: characterization and photodynamic action on bladder cancer cellsPublication . Moura, Nuno M. M.; Reis, Melani J. A.; Lodeiro, Carlos; Neves, M. Graça P. M. S.; Cavaleiro, José A. S.; Ribeiro, Carlos F.; Fernandes, Rosa; Gomes, Ana T. P. C.Cancer stands as the second leading global cause of death, following heart disease. Considering the severe side effects revealed by some chemotherapeutics for tumor treatment and anticancer therapies, the scientific community is actively exploring more effective alternatives. Photodynamic Therapy (PDT) mediated by porphyrin-based photosensitizers (PS) has emerged as an attractive alternative to more conventional therapies. In this study, we incorporated bis-cyclometalated iridium(III) complexes featuring porphyrin-arylbipyridine ligands into poly(vinylpyrrolidone) (PVP) micelle. This integration resulted in photostable PVP-PS formulations with a remarkable capability to generate singlet oxygen. These formulations were also efficiently internalized by HT-1379 cells and due to these features, their photodynamic action against human bladder cancer cells (HT-1376 cell line) was assessed. All the formulations demonstrated high photodynamic activity, with PVP-2 and PVP-3 proving to be the most promising PS, as evidenced by their lower IC50PDT values. It was also demonstrated that all PVP-based formulations provide a safe and effective approach for photodynamic therapy (PDT) in bladder cancer, as no cytotoxic effects were observed in Vero cells.
- Unraveling the photodynamic activity of cationic benzoporphyrin-based photosensitizers against bladder cancer cellsPublication . Gomes, Ana T. P. C.; Neves, M. Graça P. M. S.; Fernandes, Rosa; Ribeiro, Carlos F.; Cavaleiro, José A. S.; Moura, Nuno M. M.In this study, we report the preparation of new mono-charged benzoporphyrin complexes by reaction of the appropriate neutral benzoporphyrin with (2,2′-bipyridine)dichloroplatinum(II) and of the analogs’ derivatives synthesized through alkylation of the neutral scaffold with iodomethane. All derivatives were incorporated into polyvinylpyrrolidone (PVP) micelles. The ability of the resultant formulations to generate reactive oxygen species was evaluated, mainly the singlet oxygen formation. Then, the capability of the PVP formulations to act as photosensitizers against bladder cancer cells was assessed. Some of the studied formulations were the most active photosensitizers causing a decrease in HT-1376 cells’ viability. This creates an avenue to further studies related to bladder cancer cells.