Browsing by Author "Pinto, Miguel Marques"
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- Chemical characterization, cytotoxic evaluation and anti-SARS-CoV2 activity of plant extracts rich in hydrolysable tanninsPublication . Melo, Adma N. F.; Afonso, Tiago B.; Carvalho, Marta; Rodrigues, Cláudia; Ribeiro, Tânia; Carocho, Márcio; Pinto, Miguel Marques; Tavaria, Freni; Teixeira, Paula; Simas, J. Pedro; Barros, Lillian; Pintado, Manuela
- Evaluation of minimum inhibitory concentrations of plant extracts against environmental fungi and dermatophytesPublication . Afonso, Tiago B.; Melo, Adma N. F.; Carvalho, Marta; Ribeiro, Tânia; Pinto, Miguel Marques; Barros, Lillian; Carocho, Márcio; Tavaria, Freni; Teixeira, Paula; Pintado, Manuela
- Plant extract with bioactive potential against SARS-CoV-2Publication . Melo, Adma N. F.; Afonso, Tiago B.; Carvalho, Marta; Rodrigues, Cláudia; Ribeiro, Tânia; Carocho, Márcio; Pinto, Miguel Marques; Tavaria, Freni; Teixeira, Paula; Simas, J. Pedro; Barros, Lillian; Pintado, ManuelaBackground: The novel disease caused by SARS-CoV-2 has been causing chaos worldwide due to its high mortality, morbidity and contagiousness nature, and therefore forced the scientific world to develop new alternatives to combat and assist the fight against this virus. Plant extracts have bioactive molecules that might provide a starting point for the research on the use of plants as an excellent source of new antiviral agents able to inhibit or neutralize COVID-19. Therefore, the objective of this study was to obtain an extract from a Plant cultivated under controlled conditions in Portugal to be used as an antiviral substance to be applied against COVID-19. Materials/Methods: The plant extract obtained from plant cultivated under controlled conditions in Portugal was extracted by maceration in a hydroethanolic solution under optimized conditions and submitted to freeze drying. The extracts were evaluated for their phenolic profile by high performance liquid chromatography coupled to a diode array detector and a mass spectrometer detector (HPLC-DAD-MS). Antioxidant activity was also measured, and antiviral properties were measured for SARS-CoV-2 in Vero cells. Results. HPLC analysis revealed the presence of different phenolic compounds in the extracts with relevance for caftaric acid, cis-chicoric acid and trans-chychoric acid. The extract showed a good antioxidant capacity demonstrated by the high value found for ORAC of 3202,82 ± 32,52 Trolox/mg extract. Regarding the antiviral activity, the results achieved for the viral titer was 20 PFU/mL, and the antiviral activity for SARS-CoV-2 was 4.02 ± 0.00 Mv and the percentage of reduction was > 99.99%. Conclusion: The results showed that the obtained extract demonstrated consistent results of antiviral activity presenting a potential for applications against the SARS-CoV-2. Further studies are required for validation and application of this extract.
- Plant extract with bioactive potential against SARS-CoV-2Publication . Melo, Adma N. F.; Afonso, Tiago B.; Carvalho, Marta; Rodrigues, Cláudia; Ribeiro, Tânia; Carocho, Márcio; Pinto, Miguel Marques; Tavaria, Freni; Teixeira, Paula; Simas, J. Pedro; Barros, Lillian; Pintado, Manuela
- Red grape pomace extract: bioactive potential against bacteria, fungi and SARS-CoV-2Publication . Melo, Adma; Afonso, Tiago B.; Carvalho, Marta; Rodrigues, Cláudia; Ribeiro, Tânia; Carocho, Márcio; Pinto, Miguel Marques; Tavaria, Freni; Teixeira, Paula; Simas, J. Pedro; Barros, Lillian; Pintado, ManuelaThe outbreak of COVID-19 disease caused by SARS-CoV-2 forced the scientific world to search for new alternatives to help control the virus. Grape pomace, which is an industrial residue obtained from the winemaking process, has bioactive compounds derived from the grape [1], which can be a starting point for research on the use this residue as a source of new antiviral agents. Thus, the objective of this study was to obtain an extract from the by-product of red grapes discarded by the wine industry in Portugal to be used as an antiviral agent with possible application in disinfectants, fabrics or other materials. The red grape pomace extract was obtained by maceration in a hydroethanolic solution (ethanol:H2O 50:50 v/v) under optimized conditions and submitted to freeze drying. The extract was tested against pathogenic bacteria and a variety of fungi, and the antiviral activity was evaluated for SARS-CoV-2 in Vero cells. The extract showed antimicrobial activity against a large number of bacteria tested, e.g., B. cereus (1.56 mg/mL), L. monocytogenes and S. aureus (3.125 mg/mL), and E. coli and S. Typhimurium (50 mg/mL). Regarding its antifungal potential, this extract did not show inhibition against the environmental fungi tested, however, it inhibited two of the tested dermatophytes. T. mentagrophytes and the yeast M. furfur at a concentration of 6.25 mg/mL and 50 mg/mL, respectively. Regarding the antiviral activity, the results achieved for the viral titer was 6000 PFU/mL, the antiviral activity for SARS-CoV-2 was 1.36 ± 0.15 Mv and the percentage of reduction was of 95.38 ± 1.54%. The results showed that the obtained extract revealed consistent results of antiviral activity, presenting a potential for applications against SARS-CoV-2. In addition, it showed potential against some bacterial pathogens and fungal dermatophytes. Further studies are required for the validation and application of this extract.