Percorrer por autor "Pais, Adriana C. S."
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- Exploring the bioaccessibility and intestinal absorption of major classes of pure phenolic compounds using in vitro simulated gastrointestinal digestionPublication . Pais, Adriana C. S.; Coscueta, Ezequiel R.; Pintado, Maria Manuela; Silvestre, Armando J. D.; Santos, Sónia A. O.The bioaccessibility and bioavailability of phenolic compounds (PC) influence directly their role in disease prevention/control. Studies have evaluated this ability through complex plant and food matrices, which may reflect more a synergistic effect of the matrix than the ability of the PCs, hindering their individual exploitation in nutraceutical or pharmaceutical applications. In the present study ten pure PCs representing major classes were evaluated for their bioaccessibility and intestinal absorption in an in vitro simulated gastrointestinal digestion (SGD). This is the first study concerning the bioaccessibility evaluation of pure phloretin, phloroglucinol, naringin, naringenin and daidzein, while no in vitro SGD has been performed before for the other compounds considered here. PCs were analyzed through ultra-high-performance liquid chromatography coupled with diode-array detection and tandem mass spectrometry (UHPLC-DAD-MSn). Most of the compounds remained present along the gastrointestinal tract, and the bioaccessibility was in general higher than 50%, except for quercetin, epigallocatechin gallate, and ellagic acid. All compounds were highly absorbed in the intestine, with phloretin showing the lowest percentage at about 82%. The study findings provide new knowledge on the bioaccessibility and intestinal absorption of different PCs classes.
- Exploring the bioavailability of phenolic compounds through in vitro simulated gastrointestinal digestion: INFOGESTPublication . Pais, Adriana C. S.; Coscueta, Ezequiel R.; Pintado, Maria Manuela; Silvestre, Armando J. D.; Santos, Sónia A. O.Conclusions: Studied phenolic compounds did not undergo enzymatic digestion after INFOGEST, as we did not detect any resultant metabolites. Bioaccessibility percentages determined after the intestinal digestion phase showed that most of the studied phenolic compounds were about 50% bioaccessible. Absorption rates of all studied phenolic compounds were high (> 80%), suggesting that they can be absorbed into the systemic circulation or follow to the colon, where gut microbiota may metabolize them. Solubility of phenolic compounds could limit their bioavailability, as QUE and EA, demonstrated lower bioaccessibility along the simulated gastrointestinal tract due to their low solubility in water.
- Gut microbiota modulation induced by pure phenolic compounds: an in vitro fecal fermentation studyPublication . Pais, Adriana C. S.; Ribeiro, Tânia B.; Coscueta, Ezequiel R.; Salsinha, Ana Sofia; Pintado, Maria Manuela; Silvestre, Armando J. D.; Santos, Sónia A. O.
- Identification of novel metabolic signatures on human gut microbiota: ellagic acid, naringenin, and phloroglucinolPublication . Pais, Adriana C. S.; Ribeiro, Tânia B.; Coscueta, Ezequiel R.; Pintado, Maria Manuela; Silvestre, Armando J. D.; Santos, Sónia A. O.Phenolic compounds are widely known for their beneficial effects on human health. However, it is essential to understand which low molecular weight metabolites are produced by the gut microbiota, when non-absorbed compounds reach the colon, and whether these metabolites are more biologically active than their precursors. In this context, this study aims to explore the gut microbiota metabolites of relevant phenolic compounds commonly found in the human diet. Therefore, ellagic acid, naringenin, and phloroglucinol were incubated with human feces for 48 h, and the ensuing metabolites were analyzed by ultra-high-performance liquid chromatography with diode array detector coupled to ion trap mass spectrometry (UHPLC-DAD-MSn) and gas chromatography–mass spectrometry (GC-MS). Ellagic acid metabolism by the gut microbiota produced a diversity of urolithins, with 8-hydroxyurolithin being identified for the first time. Isomers of 4-hydroxybenzoic, 3,4-dihydroxybenozic, and p-coumaric acids were identified for the first time as naringenin metabolites, while phloroglucinic, 2-hydroxy-3-phenylpropanoic, 3-phenylpropanoic, and 2-phenylacetic acids are reported for the first time as phloroglucinol metabolites. These findings contribute to a more comprehensive understanding of the beneficial health effects of these metabolites through the evaluation of their biological activities in conjunction with their effects on the gut microbiota, thus providing the basis for the development of food supplements, novel probiotics or functional foods.
- Metabolism of phenolic compounds by human gut microbiota: insights from in vitro fecal fermentationPublication . Pais, Adriana C. S.; Ribeiro, Tânia B.; Coscueta, Ezequiel R.; Pintado, Maria M.; Silvestre, Armando J. D.; Santos, Sónia A. O.
- Phenolic compounds as modulators of gut microbiota composition: an in vitro batch fecal fermentation approachPublication . Pais, Adriana C. S.; Ribeiro, Tânia B.; Coscueta, Ezequiel R.; Salsinha, Ana Sofia; Pintado, Maria Manuela; Silvestre, Armando J.D.; Santos, Sónia A. O.The human diet comprises a variety of bioactive compounds, such as phenolic compounds which are widely present in vegetables and fruits that confer numerous health benefits.1 However, the health benefits of these compounds appear to be directly influenced by a number of factors, including their bidirectional relationship with gut microbiota,1,2 as these compounds are metabolized by these microorganisms, and, simultaneously, modulate their composition.1,3 Nevertheless, knowledge about the impact of these dietary compounds have individually on human gut microbiota remains limited 3,4. Three phenolic compounds (ellagic acid, naringenin, and phloroglucinol) were subjected to an in vitro batch fermentation with a pool of fresh human fecal samples collected from healthy donors. Subsequently, the samples were further analyzed by 16S amplicon metagenomics sequencing and their short-chain fatty acids (SCFAs) profile was determined by gas chromatography with a flame ionization detector (GC-FID). Ellagic acid and phloroglucinol exhibited prebiotic properties, as evidenced by the production of specific SCFAs, including acetic, propanoic, and butyric acids. Furthermore, these compounds positively influenced the growth of beneficial genera of bacteria, such as Lactobacillus and Bifidobacterium. In contrast, naringenin exerted an influence on other genera with a pathogenic character, such as Escherichia and Salmonella. These findings contribute significantly to our understanding of how these phenolic compounds influence the gut microbiota composition. As a future perspective, this knowledge could be relevant to the development of new formulations in the nutraceutical or pharmaceutical areas. Furthermore, studying of phenolic compounds in combination may be a crucial step in future research to achieve a more diverse and well-balanced gut microbiota ecosystem.
- Phenolic compounds' impact on gut microbiota: insights from in vitro batch fecal fermentation for composition modulationPublication . Pais, Adriana C. S.; Ribeiro, Tânia B.; Coscueta, Ezequiel R.; Salsinha, Ana Sofia; Pintado, Maria Manuela; Silvestre, Armando J. D.; Santos, Sónia A. O.The relationship between phenolic compounds and gut microbiota (has been widely studied to explore the health benefits of these bioactive dietary compounds. Phenolic compounds are metabolized by gut microbiota, while also modulating its composition. However, the individual effects of these compounds on human gut microbiota remain underexplored. To address this, three phenolic compouds—ellagic acid, naringenin, and phloroglucinol—underwent in vitro batch fermentation with fecal samples from healthy donors. Samples were analyzed through 16S metagenomics sequencing, and short-chain fatty acids (SCFAs) were measured using gas chromatography.Results showed that ellagic acid and phloroglucinol had prebiotic properties, producing SCFAs like acetic, propanoic, and butyric acids and promoting the growth of beneficial bacteria such as Lactobacillus and Bifidobacterium. In contrast, naringenin was linked to the growth of pathogenic genera like Escherichia and Salmonella. This study provides valuable insights into how specific phenolic compounds influence gut microbiota composition, contributing to potential pharmaceutical or nutraceutical developments.
- Screening of different phenolic compounds classes bioavailability and bioaccessibility, through an in vitro gastrointestinal digestion systemPublication . Pais, Adriana C. S.; Coscueta, Ezequiel R.; Pintado, Maria Manuela; Silvestre, Armando J. D.; Santos, Sónia A. O.Introduction: For centuries, plants have been seen by humans as an essential source of food or natural healthcare products.1 In an effort to improve consumers health' and well-being, much effort is being centered on the exploitation of their biological activities.2 Phenolic compounds are secondary metabolites with a huge structural diversity and widely distributed in the plant kingdom, and thus, commonly present in human diet, as part of the natural diet, or as supplements. Due to their vast range of biological activities (such as antioxidant, anti-inflammatory and/or antiproliferative) have been considered very promising in the prevention of some disorders.2-5 Notwithstanding, these human health beneficial effects could be influenced by phenolic compounds bioavailability. In fact, bioavailability could be influenced by a huge diversity of factors, namely, compound’s structure, human enzymatic activity, gut microbiota, among others.6 In this vein, this work aimed to evaluate the bioavailability and intestinal absorption of different classes of phenolic compounds through an in vitro gastrointestinal digestion. This screening allowed to determine the enzymatic-resistance of these phenolic compounds and understand the percentage of simulated intestine absorption. Conclusions: The studied phenolic compounds have been widely screened by the scientific community for their biological activities, such as antioxidant, anti-inflammatory and antiproliferative, thus, as they seem to reach the intestine intact, they may be very promising in the prevention of some intestinal diseases. Similarly, their absorption could result in human beneficial effects.